Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression of in vitro cultivated preB cells before and after 8, 16 and 24 hours induction of miR-221

ABSTRACT: PreB cells were analyzed for differences in gene expression before and after the overexpression of miR-221. In order to dissect possible targets for the miR-221, gene expression profiles of preB cells un-induced or induced for the miR-221 expression after 8, 16 and 24 hours were compared. All induction time-points, e.g. after 8, 16 and 24 hours were compared to un-induced preB cells and to each other group. Gene expression profiles of un-induced preB cells, preB cells induced for miR-221 expression after 8, 16, and 24 hours were analyzed using Affymetrix MG 430 2.0 whole genome arrays. Each time-point was performed in triplicates for un-induced preB cells and preB cells induced for miR-221 expression after 8, 16, and 24 hours (12 arrays in total). To obtain genes significantly downregulated upon induction of miR-221, the expression profiles of un-induced preB cells were compared to preB cells activated for 8, 16, or 24 h and were also compared to each other. After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 M-BM-5g cRNA hybridized in triplicates for each of the four groups to the 12 GeneChip arrays: Group1, un-induced preB cells, Group2, preB cells induced for miR-221 expression after 8h, Group3, preB cells induced for miR-221 expression after 16h, Group4, preB cells induced for miR-221 expression after 24h.

ORGANISM(S): Mus musculus

SUBMITTER: Joachim GrM-CM-<n

PROVIDER: E-GEOD-47643 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

miR-221 redirects precursor B cells to the BM and regulates their residence.

Knoll Marko M Simmons Szandor S Bouquet Corinne C Grün Joachim R JR Melchers Fritz F

European journal of immunology 20130708 9

Pluripotent hematopoietic stem cells and multipotent myeloid/lymphoid progenitors express miR-221 and miR-222. When Pax5 expression commits these progenitors to monopotent pre-B lymphocytes the two microRNAs (miRNAs) are downregulated. Upon transplantation, stem cells and progenitors can reside in the BM, while pre-B cells, after their commitment, no longer do so. Retrovirally transduced, doxycycline-induced overexpression of either miR-221 or miR-222 in pre-B-I cells does not revert their monop ...[more]

PMID: 23716169

Publication: 1/2

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Dataset Information

Gene expression of in vitro cultivated preB cells before and after 8, 16 and 24 hours induction of miR-221

Publications

miR-221 redirects precursor B cells to the BM and regulates their residence.

Similar Datasets

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