Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Rsk2 controls synovial fibroblast hyperplasia and the course of arthritis.

ABSTRACT: To understand differences in the pathogenesis of synovial hyperplasia during TNF-induced arthritis, we compared the global gene expression of hTNFtg and hTNFtg;Rsk2-/y primary synovial fibroblasts. Murine fibroblast-like synoviocytes (FLS) were isolated from the hind paws of hTNFtg and hTNFtg;Rsk2-/y (background: C57BL/6) 6-9 week-old mice via collagenase digestion. The cells of two mice were pooled and cultivated for at least 3 passages before usage. For characterization, the cells were tested for the surface expression of CD90.2 and VCAM1 by flow cytometric analysis, whereas CD11b as a monocytic marker had to be absent. Total RNA was extracted using the RNeasy Mini kit (Qiagen). The integrity and amount of isolated RNA was assessed for each sample using an Agilent 2100 Bioanalyzer (Agilent, Waldbronn, Germany) and a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). Double-stranded complementary RNA was synthesized from 1 M-BM-5g total RNA using Message AmpII Biotin (Ambion, USA). Fifteen micrograms of fragmented cRNA of each sample were hybridized to six MG_U430_2 GeneChips (Affymetrix). Hybridization was performed in a Hybridization Oven 640, and chips were washed and stained in the Fluidics Station 450 (both Affymetrix). Finally, the arrays were scanned with a GeneChip Scanner 3000 using the GCOS software, version 1.4, both Affymetrix. All relevant GCOS data of quality checked microarrays were analyzed with High Performance Chip Data Analysis (HPCDA, unpublished), using the BioRetis database (www.bioretis-analysis.de), as described and validated previously.

ORGANISM(S): Mus musculus

SUBMITTER: Joachim GrM-CM-<n

PROVIDER: E-GEOD-52992 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Rsk2 controls synovial fibroblast hyperplasia and the course of arthritis.

Derer Anja A Böhm Christina C Grötsch Bettina B Grün Joachim R JR Grützkau Andreas A Stock Michael M Stock Michael M Böhm Sybille S Sehnert Bettina B Gaipl Udo U Schett Georg G Hueber Axel J AJ David Jean-Pierre JP

Annals of the rheumatic diseases 20141120 2

<h4>Objective</h4>Arthritis is a chronic inflammatory disease characterised by immune cell infiltration and mesenchymal cell expansion in the joints. Although the role of immune cells in arthritis is well characterised, the development of mesenchymal cell hyperplasia needs to be better defined. Here, we analysed the role of the ribosomal S6 kinase Rsk2, which we found to be highly activated in joints of patients with arthritis, in the development of mesenchymal cell hyperplasia.<h4>Methods</h4>W ...[more]

PMID: 25414238

Publication: 1/2

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Dataset Information

Rsk2 controls synovial fibroblast hyperplasia and the course of arthritis.

Publications

Rsk2 controls synovial fibroblast hyperplasia and the course of arthritis.

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