Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression changes caused by YAP overexpression and Nf2 deletion in the developing mouse brain


ABSTRACT: The transcriptional coactivator YAP is the key downstream effector of the Hippo pathway. YAP overexpression in the developing mouse brain results in excessive expansion of the neural progenitor pool and severe perturbation of brain development. Nf2/Merlin is a tumor suppressor whose mutations are found in many human cancers. Loss of Merlin during brain development causes overexpansion of the neural progenitor pool. We used microarrays to identify the gene expression changes caused by YAP overexpression and Nf2 deletion. We used a double-transgenic system to overexpress YAP in the developing mouse brain by crossing mice carrying a doxycycline-dependent allele of YAP1-S127A (TetO-YAP1) with those expressing the reverse tetracycline-dependent transactivator rtTA under the control of the neural progenitor-specific Nestin promoter (Nes-rtTA) and feeding the dam with doxycycline-containing food (200 mg/kg) from E7.5. We conditionally deleted Nf2 using the telencephalon-specific Emx1-Cre.

ORGANISM(S): Mus musculus

SUBMITTER: Geoffrey Neale 

PROVIDER: E-GEOD-48078 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2013-06-19 | GSE48078 | GEO
2023-08-07 | GSE224347 | GEO
2024-08-26 | GSE263122 | GEO
2022-03-24 | GSE186189 | GEO
2019-05-04 | GSE125408 | GEO
2015-02-15 | E-GEOD-64965 | biostudies-arrayexpress
2014-07-15 | GSE56157 | GEO
2022-11-19 | GSE211459 | GEO
2021-04-14 | GSE157677 | GEO
2024-04-30 | GSE244362 | GEO