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Transcription profiling of mouse fundic mucosa in the presence/absence of gastrin and in gastrin-CCK double knockout mice

ABSTRACT: Fundic mucosa gene expression in wildtype vs gastrin knockout, gastrin replaced gastrin KO and gastrin-CCK double knockout mice; Fundic mucosal scraping RNA were isolated from WT, Gastrin Knockout, Gastrin replaced KO and Gastrin-CCK double knockout mice. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix murine 430A arrays. Comparisons between the sample groups allow the identification of genes with gastrin responsiveness and Gastrin-CCK double effect . Experiment Overall Design: 3 WT, 3 Gastrin KO, 3 Gastrin replaced KO and 3 Gastrin-CCK double Knockout fundic RNA replicates were analyzed

ORGANISM(S): Mus musculus

SUBMITTER: Linda Samuelson

PROVIDER: E-GEOD-4810 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profiling of gastrin-regulated genes in mouse stomach.

Jain Renu N RN Samuelson Linda C LC

Physiological genomics 20061114 1

Gastrin, a potent stimulator of gastric acid secretion, primarily targets the acid-secreting parietal cells and histamine-secreting enterochromaffin-like (ECL) cells in the stomach. Accordingly, gastrin-deficient (GAS-KO) mice have a severe impairment in acid secretion. The aim of this study was to characterize changes in gene expression in GAS-KO mice to identify gastrin-regulated genes and to gain insight into how gastric cell types are regulated by gastrin and acid secretion. Affymetrix micro ...[more]

PMID: 17105752

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Transcription profiling of parietal cells isolated from WT and Gastrin Knockout mice to identify genes responsive to gastrin.

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2007-08-31 | E-GEOD-2772 | biostudies-arrayexpress

Fundic mucosa gene expression in the presence/absence of gastrin and in gastrin-CCK double knockout mice.

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Project description:Fundic mucosa gene expression in the presence/absence of gastrin and in gastrin-CCK double knockout mice.

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