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Genome-wide time series analysis of gastrin induced gene expression in AR42J cells with and without protein synthesis

ABSTRACT: Genome-wide gene expression time series experiments are an important approach to studying a cells response to a given stimulus, as they enable capturing of the dynamics of the response. Moreover, information about the dynamics of the system is an important prerequisite for inferences on interactions between its components. We have performed extensive microarray-based genome-wide gene expression analyses up to 14h of the response to gastrin in AR42J adenocarcinoma cells. The gastrin reponse was also investigated in presence of the protein translation inhibitor cycloheximide. The rat cell line AR42J derived from pancreatic duct has the feature of pluripotency of the precursor cells of the gut endoderm. Furthermore, AR42J cells express gastrin receptors endogenously and are therefore frequently used as a model system to study gastrin responses. The hormone gastrin is the central regulator of gastric acid secretion and in addition plays a prominent role in regulation of growth and differentiation of gastric and colonic mucosa. Hypergastrinaemia has been linked to development of neuroendocrine gastrointestinal tumours (NE-GI) and, in conjugation with additional factors such as inflammation, to development of gastric adenocarcinoma (GA)

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE32869 | GEO | 2011/10/17

SECONDARY ACCESSION(S): PRJNA146557

REPOSITORIES: GEO

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Genome-wide time series analysis of gastrin induced gene expression in AR42J cells with and without protein synthesis

Project description:Genome-wide gene expression time series experiments are an important approach to studying a cells response to a given stimulus, as they enable capturing of the dynamics of the response. Moreover, information about the dynamics of the system is an important prerequisite for inferences on interactions between its components. We have performed extensive microarray-based genome-wide gene expression analyses up to 14h of the response to gastrin in AR42J adenocarcinoma cells. The gastrin reponse was also investigated in presence of the protein translation inhibitor cycloheximide. The rat cell line AR42J derived from pancreatic duct has the feature of pluripotency of the precursor cells of the gut endoderm. Furthermore, AR42J cells express gastrin receptors endogenously and are therefore frequently used as a model system to study gastrin responses. The hormone gastrin is the central regulator of gastric acid secretion and in addition plays a prominent role in regulation of growth and differentiation of gastric and colonic mucosa. Hypergastrinaemia has been linked to development of neuroendocrine gastrointestinal tumours (NE-GI) and, in conjugation with additional factors such as inflammation, to development of gastric adenocarcinoma (GA) Times series up to 14h treated with gastrin, gastrin and cycloheximide, cycloheximide and control at individual timepoints

2011-10-16 | E-GEOD-32869 | biostudies-arrayexpress

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Project description:Fundic mucosa gene expression in wildtype vs gastrin knockout, gastrin replaced gastrin KO and gastrin-CCK double knockout mice Fundic mucosal scraping RNA were isolated from WT, Gastrin Knockout, Gastrin replaced KO and Gastrin-CCK double knockout mice. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix murine 430A arrays. Comparisons between the sample groups allow the identification of genes with gastrin responsiveness and Gastrin-CCK double effect . Keywords: repeat

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Project description:We are studying the role of PAI-1 in hypergastrinemic transgenic mice (PAI-1 knockout, HK-ATPase beta knockout and corresponding control strains). As a model of hypergastrinaemia, HK-ATPase beta knockout mice are preferred instead of giving a high dose of proton pump inhibitors daily. We have successfully crossed these two strains and generated double knockout mice (PAI-1--/HK-ATPase--) and 3 different control groups (PAI-1++/HK-ATPase++, PAI-1--/HK-ATPase++ and PAI-1++/HK-ATPase--) (9 animals in each group). The gastric phenotype of all mice has been examined by morphologic and molecular analysis after 12 months. Gastric biopsies and blood samples have been taken for histology, large scale gene expression analysis, verification of mRNA and protein expression and gastrin measurements. Global gene expression analysis has been done on gastric mucosal samples from all groups of mice. The differences between wild type mice and the transgenic animals are expected to provide novel hypotheses on the role of PAI-1 in gastrin-induced changes in the gastric mucosa.

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Project description:Parietal cells were isolated from WT and Gastrin Knockout mice. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix murine U74Av2 arrays. Comparisons between the sample groups allow the identification of genes with gastrin responsiveness. Experiment Overall Design: 3 WT parietal cell and 3 Gastrin Kncokout parietal cell replicates were analyzed

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