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Set1, H3K4A, set1htz1, set1htz1sir2 transcription profiles

ABSTRACT: Relative mRNA abundance of all S. cerevisiae genes was measured in various mutants, compared to wild-type mutant and wild-type grown to log phase, YPAD, 30C, purified mRNA, reverse-transcribed to cDNA, labelled with Cy5/Cy3, hybridized to whole genome ORF microarrays

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Shivkumar Venkatasubrahmanyam

PROVIDER: E-GEOD-4826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide, as opposed to local, antisilencing is mediated redundantly by the euchromatic factors Set1 and H2A.Z.

Venkatasubrahmanyam Shivkumar S Hwang William W WW Meneghini Marc D MD Tong Amy Hin Yan AH Madhani Hiten D HD

Proceedings of the National Academy of Sciences of the United States of America 20071009 42

In Saccharomyces cerevisiae, several nonessential mechanisms including histone variant H2A.Z deposition and transcription-associated histone H3 methylation antagonize the local spread of Sir-dependent silent chromatin into adjacent euchromatic regions. However, it is unclear how and where these factors cooperate. To probe this question, we performed systematic genetic array screens for gene deletions that cause a synthetic growth defect in an htz1Delta mutant but not in an htz1Delta sir3Delta do ...[more]

PMID: 17925448

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