Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profile of abf1-1 mutant at 36 degrees C


ABSTRACT: The abf1-1 mutant and wild type cells were grown at 30 degrees C in YPAD until OD600 reached 1.0-1.2. The same volume of 42 degrees C preheated YPAD was added for each flask. After 45 min incubation at 36 degrees C, cells were harvested and RNA was isolated. Four repeats of microarray results are in this series.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Rong Li 

PROVIDER: E-GEOD-1492 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide analysis of ARS (autonomously replicating sequence) binding factor 1 (Abf1p)-mediated transcriptional regulation in Saccharomyces cerevisiae.

Miyake Tsuyoshi T   Reese Justin J   Loch Christian M CM   Auble David T DT   Li Rong R  

The Journal of biological chemistry 20040610 33


Autonomously replicating sequence-binding factor-1 (Abf1p) is an essential sequence-specific transcription factor in Saccharomyces cerevisiae that participates in multiple nuclear events including DNA replication, transcription activation, and gene silencing. Numerous gene-specific analyses have implicated Abf1p in the transcriptional control of genes involved in a diverse range of cellular functions, leading to the notion that Abf1p acts as a global transcriptional regulator. Here we report fin  ...[more]

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