Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

The effect of chronic Rapamycin on the transcriptome of old mouse liver

ABSTRACT: Analysis of the effect of gene expression in the livers of old mice (25 months of age) fed rapamycin chronically (21 months) from 4 months of age. Total RNA extracted from livers of 25 month old C57BL6/J male and female mice started on control or 14 ppm rapamycin (Rapa) from 4 months of age on 21 months of treatment. Number of samples total: 69, with 19 samples in Control males, 13 samples in Rapa males, 16 samples in Control Females, and 21 samples in Rapa Females

ORGANISM(S): Mus musculus

SUBMITTER: Wilson Fok

PROVIDER: E-GEOD-48333 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mice fed rapamycin have an increase in lifespan associated with major changes in the liver transcriptome.

Fok Wilson C WC Chen Yidong Y Bokov Alex A Zhang Yiqiang Y Salmon Adam B AB Diaz Vivian V Javors Martin M Wood William H WH Zhang Yongqing Y Becker Kevin G KG Pérez Viviana I VI Richardson Arlan A

PloS one 20140107 1

Rapamycin was found to increase (11% to 16%) the lifespan of male and female C57BL/6J mice most likely by reducing the increase in the hazard for mortality (i.e., the rate of aging) term in the Gompertz mortality analysis. To identify the pathways that could be responsible for rapamycin's longevity effect, we analyzed the transcriptome of liver from 25-month-old male and female mice fed rapamycin starting at 4 months of age. Few changes (<300 transcripts) were observed in transcriptome of rapamy ...[more]

PMID: 24409289

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Project description:The effect of pre and periconceptional multiple micronutrient supplementation on methylation of CpG loci within CpG islands associated with 14,000 genes across the human genome has been investigated in the offspring of a cohort of Gambian women participating in a controlled double blinded trial. Methylation levels in placebo and micronutrient supplemented cohorts were compared in genomic DNA from cord blood (35 placebo and 21 micronutrient supplemented) and in circulating blood samples (14 placebo and 9 micronutrient supplemented) drawn from the same cohort at 9 months old. A small number of differentially methylated CpG loci were identified in cord blood (14 in males and 21 in females) and a larger number in the 9 month infant comparison (108 in males and 106 in females). Seven differentially methylated loci in males and 8 in females persisted from cord to infant. These findings indicate that micronutrient supplementation pre-conception or early in embryonic development is potentially associated with programming of gene activity at birth, which is maintained into early infanthood. Strikingly, the loci affected by micronutrient supplementation differed between males and females, with no shared changes in cord blood and only 5 shared changes at 9 months. Additionally, a large number of CpG loci showed variation in methylation level when comparing 9-month samples to cord blood samples. These postnatal changes were more consistent between sexes, with 85% of female alterations being found as a subset of male changes in the placebo cohort and 62% of the female changes shared with males in the supplemented cohort. Taken together, the results suggest that there is a core developmental program shared between the sexes that is unaffected by nutrient supplementation, but that there are also sex-specific developmental changes which are altered under conditions of micronutrient supplementation and deficiency. Bisulphite converted DNA from 59 cord (36 placebo and 23 treated) and 25 infant peripheral blood (15 placebo and 10 treated) samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2. (For further analysis, five were excluded during quality control leaving 56 cord (35 placebo and 21 treated) and 23 infant peripheral blood (14 placebo and 9 treated) samples. Full data set of 84 samples were submitted to GEO)

Dataset Information

The effect of chronic Rapamycin on the transcriptome of old mouse liver

Publications

Mice fed rapamycin have an increase in lifespan associated with major changes in the liver transcriptome.

Similar Datasets

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