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Short-term rapamycin treatment in mice have few effects on the transcriptome of white adipose tissue compared to dietary restriction

ABSTRACT: Analysis of the transcriptome in the epididymal fat of young mice (8 months of age) from treatment of dietary restriction, or rapamycin Total RNA extracted from epididymal fat of 8 month old C57BL6/J mice started on ad libitum (AL) , 40% dietary restriction (DR), or 14 ppm rapamycin (Rapa) from 2 months of age on 6 months of treatment. Number of samples total: 24, with 8 samples in AL, 8 samples in DR, and 8 samples in Rapa

ORGANISM(S): Mus musculus

SUBMITTER: Wilson Fok

PROVIDER: E-GEOD-52825 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction.

Fok Wilson C WC Livi Carolina C Bokov Alex A Yu Zhen Z Chen Yidong Y Richardson Arlan A Pérez Viviana I VI

Mechanisms of ageing and development 20140727

Rapamycin, a drug that has been shown to increase lifespan in mice, inhibits the target of rapamycin (TOR) pathway, a major pathway that regulates cell growth and energy status. It has been hypothesized that rapamycin and dietary restriction (DR) extend lifespan through similar mechanisms/pathways. Using microarray analysis, we compared the transcriptome of white adipose tissue from mice fed rapamycin or DR-diet for 6 months. Multidimensional scaling and heatmap analyses showed that rapamycin ha ...[more]

PMID: 25075714

Similar Datasets

Short-term rapamycin treatment in mice have few effects on the transcriptome of white adipose tissue compared to dietary restriction

Project description:Analysis of the transcriptome in the epididymal fat of young mice (8 months of age) from treatment of dietary restriction, or rapamycin

2014-08-18 | GSE52825 | GEO

Combined treatment of rapamycin and dietary restriction has a larger effect on the transcriptome and metabolome of liver

Project description:Rapamycin (Rapa) and dietary restriction (DR) have consistently been shown to increase lifespan. To investigate whether Rapa and DR affect similar pathways in mice, we compared the effects of feeding mice ad libitum (AL), Rapa, DR, or a combination of Rapa and DR (Rapa + DR) on the transcriptome and metabolome of the liver. The principal component analysis shows that Rapa and DR are distinct groups. Over 2500 genes are significantly changed with either Rapa or DR when compared with mice fed AL; more than 80% are unique to DR or Rapa. A similar observation was made when genes were grouped into pathways; two-thirds of the pathways were uniquely changed by DR or Rapa. The metabolome shows an even greater difference between Rapa and DR; no metabolites in Rapa-treated mice were changed significantly from AL mice, whereas 173 metabolites were changed in the DR mice. Interestingly, the number of genes significantly changed by Rapa + DR when compared with AL is twice as large as the number of genes significantly altered by either DR or Rapa alone. In summary, the global effects of DR or Rapa on the liver are quite different and a combination of Rapa and DR results in alterations in a large number of genes and metabolites that are not significantly changed by either manipulation alone, suggesting that a combination of DR and Rapa would be more effective in extending longevity than either treatment alone.

2013-11-08 | GSE40977 | GEO

The effect of short term Rapamycin on the transcriptome of old mouse liver

Project description:Analysis of the effect of gene expression in the livers of old mice (25 months of age) fed rapamycin short term (6 months) Rapamycin from 19 months of age. Total RNA extracted from livers of 25 month old C57BL6/N male and female mice started on control or 14 ppm rapamycin (Rapa) from 19 months of age on 6 months of treatment. Number of samples total: 42, with 10 samples in Control males, 12 samples in Rapa males, 9 samples in Control Females, and 11 samples in Rapa Females

2014-02-07 | E-GEOD-48331 | biostudies-arrayexpress

The effect of chronic Rapamycin on the transcriptome of old mouse liver

Project description:Analysis of the effect of gene expression in the livers of old mice (25 months of age) fed rapamycin chronically (21 months) from 4 months of age. Total RNA extracted from livers of 25 month old C57BL6/J male and female mice started on control or 14 ppm rapamycin (Rapa) from 4 months of age on 21 months of treatment. Number of samples total: 69, with 19 samples in Control males, 13 samples in Rapa males, 16 samples in Control Females, and 21 samples in Rapa Females

2014-02-07 | E-GEOD-48333 | biostudies-arrayexpress

Disentangling mTOR signalling from the lifespan extending effect of dietary restriction in progeroid DNA-repair deficient mice

Project description:Dietary restriction (DR) and rapamycin, the best-known DR-mimetic, are two intervention that extend health- and lifespan across multiple species. We have recently shown that DR works extremely well in progeroid DNA repair deficient mice and dramatically extend their lifespan by ~200%. We next questioned the applicability of rapamycin in these mouse mutants. Here we show that treatments with rapamycin did significantly lower mTOR-signalling but did not improve the lifespan of DNA repair deficient Ercc1{delta}/- mice. These data are an extended version of the data in GEO Series GSE77321

2021-01-25 | GSE149029 | GEO

Single-nuclei sequencing of the mouse hippocampus of dietary restricted mice

Project description:Dietary restriction (DR), reduced food intake while avoiding malnutrition, profoundly extends lifespan in most model and non-model organisms. Both chronic (i.e. life-long) and acute (i.e. late-onset) DR have been shown to improve cognitive performance in aged mice compared to animals with an unrestricted access to food (ad libitium feeding; AL). Yet so far, quantitative analyses of the molecular dynamics in the brain of DR fed animals have been limited. Here we performed single-nuclei sequencing (Nuc-seq) of whole hippocampus isolated from young (5 months) and old (24 months) AL fed animals, as well as old chronic DR (DR started at 3 months) and acute DR (aDR) mice.

2023-04-17 | GSE227515 | GEO

High-fat dietary restriction in mice induces substrate efficiency and improves metabolic health

Project description:High energy intake and, specifically, high dietary fat intake challenges the mammalian metabolism and correlates with many metabolic disorders, such as obesity and diabetes. Dietary restriction (DR) is, on the other hand, known to prevent the development of metabolic disorders. The current Western diets are highly enriched in fat and it is as yet unclear whether DR on a certain high-fat (HF) diet elicits similar beneficial effects on health. Here, we report that HF-DR improves metabolic health of mice, compared to mice receiving the same diet on an ad-libitum basis (HF-AL). Already after five weeks of restriction the serum levels of cholesterol and leptin were significantly decreased in HF-DR mice, while their glucose sensitivity and serum adiponectin levels were increased. The body weight and measured serum parameters remained stable in the following 7 weeks of restriction, implying metabolic adaptation. To understand the molecular events associated with this adaptation, we analysed gene expression in white adipose tissue (WAT) with whole genome microarrays. HF-DR strongly influenced gene expression in WAT; in total 8,643 genes were differentially expressed between both groups of mice, with a major role for genes involved in lipid metabolism and mitochondrial functioning. This was confirmed by qRT-PCR and substantiated by an increase in mitochondrial density in WAT of HF-DR mice. These results provide new insights in the metabolic flexibility of dietary restricted animals and suggest the development of substrate efficiency. Limiting food intake by decreasing portion sizes, while maintaining energy sufficiency, may similarly benefit metabolic health in humans.

2011-08-24 | GSE27213 | GEO

Intra-myocellular fatty acid synthesis and breakdown plays a critical role in mediating responses to dietary restriction in Drosophila melanogaster

Project description:Dietary restriction (DR) is a robust environmental intervention that slows aging in various species. Changes in fat content have been associated with DR, but whether they play a causal role in mediating various responses to DR remains unknown. We demonstrate that upon DR, Drosophila melanogaster shift their metabolism towards increasing both fatty acid synthesis and breakdown. Inhibition of acetyl CoA carboxylase (ACC), a critical enzyme in fatty acid synthesis, or fatty acid oxidation genes specifically in the muscle tissue inhibited the lifespan extension observed upon DR, suggesting a critical role for intra-myocellular fatty acid metabolism. DR enhances spontaneous activity of flies which was found to be dependent on the enhanced fatty acid metabolism. Furthermore, this increase in activity upon DR was found to partially mediate the lifespan extension upon DR. Over-expression of adipokinetic hormone (dAKH) in whole flies, which increases fat metabolism, led to an increase in spontaneous activity and lifespan in a nutrient dependent manner. Together these results suggest that in Drosophila melanogaster enhanced fat metabolism in the muscle is a key metabolic adaptation in response to DR. 24 experimental samples were analyzed using Nimblegen oligo microarrays. Wild type samples (AL without RU486) were used as the Cy3 reference/control for all experimetal comparisons.

2012-06-14 | E-GEOD-37537 | biostudies-arrayexpress

Intra-myocellular fatty acid synthesis and breakdown plays a critical role in mediating responses to dietary restriction in Drosophila melanogaster

2012-06-15 | GSE37537 | GEO

Expression data from germ-free and conventional mice fed lard or fish oil for 11 weeks

Project description:Dietary lipids and gut microbiota may both influence adipose tissue physiology. By feeding conventional and germ-free mice high fat diets with different lipid compositon we aimed to investigate how dietary lipids and the gut microbiota interact to influence inflammation and metabolism in epididymal adipiose tissue (EWAT) Wild-type C57Bl/6 male mice 11 weeks of age were fed isocaloric diets (45% kcal fat) with either menhaden fish oil (Research Diets, D05122102) or lard (Research Diets, D10011202) for 11 weeks. Epididymal WAT samples were harvested at the end of the experiment and analyzed by microarray.

2015-07-14 | E-GEOD-70922 | biostudies-arrayexpress

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