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Expression of Sodalis glossinidius genes derived from self-cleared and infected Glossina palpalis gambiensis flies

ABSTRACT: Transcriptome analysis of Sodalis glossinidius derived from Trypanosoma brucei gambiense infection self cleared and infected Glossina palpalis gambiensis. At 3 time points (3, 10 and 20 days) after infectived blood meal, flies were analysed by PCR to isolate the infected and infection self cleared flies. Then, infected and infection self cleared flies midgut were dissected for RNA extraction. Total RNAs were extracted at 3 time points (3, 10 and 20 days) from 24 samples including, for each time, 4 infected and 4 infection self-cleared flies.

ORGANISM(S): Sodalis glossinidius

SUBMITTER: Anne Geiger 

PROVIDER: E-GEOD-48361 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The transcriptional signatures of Sodalis glossinidius in the Glossina palpalis gambiensis flies negative for Trypanosoma brucei gambiense contrast with those of this symbiont in tsetse flies positive for the parasite: possible involvement of a Sodalis-hosted prophage in fly Trypanosoma refractoriness?

Hamidou Soumana Illiassou I   Loriod Béatrice B   Ravel Sophie S   Tchicaya Bernadette B   Simo Gustave G   Rihet Pascal P   Geiger Anne A  

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 20140315

Tsetse flies, such as Glossina palpalis gambiensis, are blood-feeding insects that could be subverted as hosts of the parasite Trypanosoma brucei gambiense: initiated in the tsetse fly mid gut, the developmental program of this parasite further proceeds in the salivary glands. The flies act as vectors of this human-invasive parasite when their salivary glands sustain the generation of metacyclic trypomastigotes, the exclusive morphotypes pre-programmed to further develop in the human individuals  ...[more]

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