Unknown,Transcriptomics,Genomics,Proteomics

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Expression of Sodalis glossinidius genes derived from self-cleared and control Glossina palpalis gambiensis flies


ABSTRACT: Transcriptome analysis of Sodalis glossinidius derived from uninfected (controls) and Trypanosoma brucei gambiense infection self cleared Glossina palpalis gambiensis. 10 days after infectived blood meal, flies anal drop were analysed by PCR to isolate the infected self cleared flies. Then, uninfected (controls) and infection self cleared 10 days-flies midgut were dissected for RNA extraction. Total RNAs were extracted from 8 samples including: 4 control and 4 infection self-cleared flies.

ORGANISM(S): Sodalis glossinidius

SUBMITTER: Anne Geiger 

PROVIDER: E-GEOD-48360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies.

Hamidou Soumana Illiassou I   Tchicaya Bernadette B   Loriod Béatrice B   Rihet Pascal P   Geiger Anne A  

Frontiers in microbiology 20140527


Sodalis glossinidius, one of the three tsetse fly maternally inherited symbionts, was previously shown to favor fly infection by trypanosomes, the parasites causing human sleeping sickness. Among a population of flies taking a trypanosome-infected blood meal, only a few individuals will acquire the parasite; the others will escape infection and be considered as refractory to trypanosome infection. The aim of the work was to investigate whether fly refractoriness could be associated with specific  ...[more]

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