Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression profiling of LT-HSCs and ST-HSCs conditionally expressing MLL-ENL


ABSTRACT: We have developed a new conditional transgenic mouse showing that MLL-ENL, at an endogenous-like expression level, induces leukemic transformation selectively in LT-HSCs. To investigate the molecular mechanism of leukemic transformation in LT-HSCs conditionally expressing MLL-ENL, we preliminarily performed comprehensive gene expression profiling of CreER-transduced LT-HSCs and ST-HSCs using cDNA microarray analysis. For initial screening of candidate genes invloved in the leukemic transformation, total RNA was extracted from colony-forming cells derived from LT-HSCs and ST-HSCs transduced with CreER or mock. Four samples were analyzed, and CreER-transduced LT/ST-HSC-derived cells were compared with mock-transduced LT/ST-HSC-derived cells, while CreER/mock-transduced LT-HSC-derived cells were compared with CreER/mock-transduced ST-HSC-derived cells.

ORGANISM(S): Mus musculus

SUBMITTER: Tetsuya Nosaka 

PROVIDER: E-GEOD-48539 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Oncogenic transformation requires unlimited self-renewal. Currently, it remains unclear whether a normal capacity for self-renewal is required for acquiring an aberrant self-renewal capacity. Our results in a new conditional transgenic mouse showed that a mixed lineage leukemia (MLL) fusion oncogene, MLL-ENL, at an endogenous-like expression level led to leukemic transformation selectively in a restricted subpopulation of hematopoietic stem cells (HSCs) through upregulation of promyelocytic leuk  ...[more]

Similar Datasets

2013-07-17 | GSE48539 | GEO
2020-10-23 | GSE141206 | GEO
2024-05-08 | GSE229484 | GEO
2017-06-05 | GSE84959 | GEO
| PRJNA210504 | ENA
2023-04-30 | GSE173158 | GEO
2019-01-17 | GSE125211 | GEO
2024-04-25 | GSE265789 | GEO
2021-03-25 | GSE148883 | GEO
| EGAS00001004969 | EGA