Fate changes leading to multipotency of isolated mesenchymal cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Ofer Shoshani
PROVIDER: E-GEOD-48547 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:Mesenchymal populations include a fraction of cells exhibiting multipotency as well as others with limited differentiation range. It has been assumed that the mesenchymal cellular cascade is topped by a multipotent cell, which gives rise to progeny with diminishing differentiation potentials. Here we show that cultured mesenchymal cells, a priori exhibiting a limited differentiation potential, may gain new capacities and become multipotent following single cell isolation. These fate changes were accompanied by up-regulation of differentiation promoting genes, many of which also became H4K20me1 methylated. Early events in the process included TGFβ and Wnt modulation, and down-regulation of hypoxia signaling. Indeed, hypoxic conditions inhibited the observed cell changes. Overall, cell isolation from neighboring partners caused major molecular changes and particularly, a newly established epigenetic state. It is suggested that MSCs behave non-deterministically and non-hierarchically and should therefore be defined primarily by their capacity to undergo fate changes triggered by environmental cues. MSC 21 population and one of its derived clones (4L.1.4) were grown to confuence, and IP of fragmented DNA using an antibody directed against H4K20ME1, or non-immune serum (control) was done. Pulled DNA was then sequenced.
2014-03-20 | E-GEOD-48544 | biostudies-arrayexpress
Project description:Mesenchymal populations include a fraction of cells exhibiting multipotency as well as others with limited differentiation range. It has been assumed that the mesenchymal cellular cascade is topped by a multipotent cell, which gives rise to progeny with diminishing differentiation potentials. Here we show that cultured mesenchymal cells, a priori exhibiting a limited differentiation potential, may gain new capacities and become multipotent following single cell isolation. These fate changes were accompanied by up-regulation of differentiation promoting genes, many of which also became H4K20me1 methylated. Early events in the process included TGFβ and Wnt modulation, and down-regulation of hypoxia signaling. Indeed, hypoxic conditions inhibited the observed cell changes. Overall, cell isolation from neighboring partners caused major molecular changes and particularly, a newly established epigenetic state. It is suggested that MSCs behave non-deterministically and non-hierarchically and should therefore be defined primarily by their capacity to undergo fate changes triggered by environmental cues. The gene expression profile of dense and sparse MSC cultures in three sequential days after seeding was compared
2014-03-20 | E-GEOD-48546 | biostudies-arrayexpress
Project description:Mesenchymal populations include a fraction of cells exhibiting multipotency as well as others with limited differentiation range. It has been assumed that the mesenchymal cellular cascade is topped by a multipotent cell, which gives rise to progeny with diminishing differentiation potentials. Here we show that cultured mesenchymal cells, a priori exhibiting a limited differentiation potential, may gain new capacities and become multipotent following single cell isolation. These fate changes were accompanied by up-regulation of differentiation promoting genes, many of which also became H4K20me1 methylated. Early events in the process included TGFβ and Wnt modulation, and down-regulation of hypoxia signaling. Indeed, hypoxic conditions inhibited the observed cell changes. Overall, cell isolation from neighboring partners caused major molecular changes and particularly, a newly established epigenetic state. It is suggested that MSCs behave non-deterministically and non-hierarchically and should therefore be defined primarily by their capacity to undergo fate changes triggered by environmental cues. The gene expression profile of MSC population was compared with one of its derived single cell clones
2014-03-20 | E-GEOD-48545 | biostudies-arrayexpress
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2020-08-21 | PXD015767 | Pride
Project description:Helicobacter pylori, a gastroenteric pathogen believed to have co-evolved with humans for 100.000 years, has a high genetic variability that motivates the study of different H. pylori populations and the diseases caused by them in order to find determinants for disease evolution. In this study we utilized both genomic and proteomic tools to compare a H. pylori strain (Nic25_A) from Nicaragua isolated from a patient with intestinal metaplasia with the P12 strain from Europe isolated from a patient with duodenal ulcer. Differences in the protein expression levels between the two strains were determined by both label-free quantification (MaxQuant) and labelling methods (tandem mass tags, TMT), utilizing a lipid-based protein immobilization (LPI™) technique to target surface protein peptides. Using the MaxQuant software, we found 52 proteins significantly differing between the two strains (up-or-down regulated by a factor of 1.5) and with TMT we were able to find 18 proteins with different expression levels between the strains. The P12 (duodenal ulcer) strain had higher expression of genes belonging to the cagPAI operon, while Nic25_A (intestinal metaplasia) had higher expression of the acid response regulator ArsR, as well as proteins regulated by ArsR; KatA, AmiE, and proteins involved in urease production. The results show that differences in protein expression can be detected by proteomic approaches in H. pylori strains of different pathogenicity, which might have implications for studies of disease progression.
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