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Tet2-mediated DNA demethylation controls T cell cytokine expression [array]

ABSTRACT: The conversion of 5-methylcytosine (5mC) into 5-Hydroxymethylcytosine (5hmC) by ten-eleven translocation (Tet) family has recently been identified as a key process for active DNA demethylation, whose effects in the immune response is currently unknown. We used microarrays to characterize the regulation of Tet2 in T cells. We found that deletion of the Tet2 gene in T cells decreased expression of effector cytokines such as IFN-?, IL-17, and IL-10. To analyze the regulation of Tet2 in Th subset differentation, CD2(Cre)Tet2(f/f) mice were used to derive Tet2-deficient Th1 and Th17 cells, and Tet2(f/f) mice were used for Tet2-enriched Th1 and Th17 cells.

ORGANISM(S): Mus musculus

SUBMITTER: Kenji Ichiyama

PROVIDER: E-GEOD-48657 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of T cell cytokine expression by Tet2-mediated DNA demethylation [gene expression]

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