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Transcription profiling time series of the response of Rattus norvegicus liver to treatment with methylprednisolone

ABSTRACT: Summary: The liver is the major site of gluconeogenesis, fat processing and distribution, as well as drug and xenobiotic metabolism. Altered gene expression in the liver is centrally invovled in both the immuosuppressive and the energetic actions of corticosteroids. Hypothesis: That pharmacodynamics can be derived from expression profiling data. Specific Aim: The aim of this project is to identify distinct temporal patters of RNA expression in the liver of rats following a bolus dose of the corticosteroid methylprednisolone. 47 RG_U34A chips were used for 17 time points.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Eric Hoffman

PROVIDER: E-GEOD-487 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Modeling of corticosteroid pharmacogenomics in rat liver using gene microarrays.

Jin Jin Y JY Almon Richard R RR DuBois Debra C DC Jusko William J WJ

The Journal of pharmacology and experimental therapeutics 20030613 1

Corticosteroid (CS) pharmacogenomics was studied using gene microarrays in rat liver. Methylprednisolone (MPL) was administered intravenously at 50 mg/kg. Rats were sacrificed and liver excised at 17 time points over 72 h. RNAs from individual livers were used to query Affymetrix GeneChips that contain sequences for 8000 genes. Cluster analysis revealed six temporal patterns consisting of 197 CS-responsive probes representing 143 genes. Based on our fifth-generation model of CS pharmacokinetics/ ...[more]

PMID: 12808002

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