Unknown,Transcriptomics,Genomics,Proteomics

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Response to low dose ionizing radiation in male mouse


ABSTRACT: Male mice 10-14 weeks of age were exposed in vivo to 10 rad X-ray. The objective is to extract information on gene networks from the microarray data. Control mice were sham-exposed. Spleen was harvested 3.5 hrs post-irradiation. Each hybridization consists of one control mouse and one exposed mouse randomly paired to form a biological replicate. Each biological replicate was hybridized in duplicate, incorporating a dye swap to control for dye-specific effects.

ORGANISM(S): Mus musculus

SUBMITTER: Yutao Liu 

PROVIDER: E-GEOD-4880 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Extracting gene networks for low-dose radiation using graph theoretical algorithms.

Voy Brynn H BH   Scharff Jon A JA   Perkins Andy D AD   Saxton Arnold M AM   Borate Bhavesh B   Chesler Elissa J EJ   Branstetter Lisa K LK   Langston Michael A MA  

PLoS computational biology 20060701 7


Genes with common functions often exhibit correlated expression levels, which can be used to identify sets of interacting genes from microarray data. Microarrays typically measure expression across genomic space, creating a massive matrix of co-expression that must be mined to extract only the most relevant gene interactions. We describe a graph theoretical approach to extracting co-expressed sets of genes, based on the computation of cliques. Unlike the results of traditional clustering algorit  ...[more]

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