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Transcript profiling of ERF115 transgenic Arabidopsis thaliana

ABSTRACT: This experiment was set up in order to identify the (direct) transcriptional targets of the Ethylene Response Factor 115 (ERF115) transcription factor. Because ERF115 expression occurs in quiescent center (QC) cells and strong effects on the QC cells were observed in ERF115 overexpression plants, root tips were harvested for transcript profiling in order to focus on root meristem and QC specific transcriptional targets. Wild-type (Col-0 ecotype), erf115 mutant (SALK_021981) and ERF115 overexpressing (p35S:ERF115 ORF) root tips (three replicates each) were harvested and subjected to transcript profiling, using the Col-0 samples as control reference.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Joke Allemeersch

PROVIDER: E-GEOD-48836 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ERF115 controls root quiescent center cell division and stem cell replenishment.

Heyman Jefri J Cools Toon T Vandenbussche Filip F Heyndrickx Ken S KS Van Leene Jelle J Vercauteren Ilse I Vanderauwera Sandy S Vandepoele Klaas K De Jaeger Geert G Van Der Straeten Dominique D De Veylder Lieven L

Science (New York, N.Y.) 20131024 6160

The quiescent center (QC) plays an essential role during root development by creating a microenvironment that preserves the stem cell fate of its surrounding cells. Despite being surrounded by highly mitotic active cells, QC cells self-renew at a low proliferation rate. Here, we identified the ERF115 transcription factor as a rate-limiting factor of QC cell division, acting as a transcriptional activator of the phytosulfokine PSK5 peptide hormone. ERF115 marks QC cell division but is restrained ...[more]

PMID: 24158907

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