ShRNA profiling of human Hep2G cells knocked down for SRC-1 expressio to investigate the role of coactivator SRC-1/NcoA-1 for IL-6 target gene induction
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ABSTRACT: This experiment series addresses the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. For that purpose, HepG2 human hepatocellular carcinoma cells were manipulated to stably express an shRNA that knocks down SRC-1 expression yielding the HepG2-âSrc1 cells. Either unmanipulated HepG2 or HepG2-âSrc1 cells were then treated for various periods with IL-6. Experiment Overall Design: HepG2 or HepG2-âSrc1 (with silenced SRC-1/NcoA-1 expression) cells were either left untreated or treated with 10 ng/ml IL-6 for 1 or 4 hours. Every experiment was carried out in replicate.
ORGANISM(S): Homo sapiens
SUBMITTER: Friedemann Horn
PROVIDER: E-GEOD-4885 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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