Unknown,Transcriptomics,Genomics,Proteomics

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Normal blood cell subtypes


ABSTRACT: We used antibodies coupled to magnetic beads (MACS) to purify cell types out of whole blood from healthy donors (CD4+ T cells, CD8+ T cells, and B cells). We also profiled whole blood (collected in Pax tubes) and Peripheral Blood Mononuclear cells obtained by Ficoll-Paque based separation. RNA was extracted using Trizol, amplified linearly twice, labeled with Cy5 and co-hybridized with amplified Stratagene human reference (labeled with Cy3). A reference experiement design type is where all samples are compared to a common reference. Using regression correlation

ORGANISM(S): Homo sapiens

SUBMITTER: Chana Palmer 

PROVIDER: E-GEOD-4889 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cell-type specific gene expression profiles of leukocytes in human peripheral blood.

Palmer Chana C   Diehn Maximilian M   Alizadeh Ash A AA   Brown Patrick O PO  

BMC genomics 20060516


<h4>Background</h4>Blood is a complex tissue comprising numerous cell types with distinct functions and corresponding gene expression profiles. We attempted to define the cell type specific gene expression patterns for the major constituent cells of blood, including B-cells, CD4+ T-cells, CD8+ T-cells, lymphocytes and granulocytes. We did this by comparing the global gene expression profiles of purified B-cells, CD4+ T-cells, CD8+ T-cells, granulocytes, and lymphocytes using cDNA microarrays.<h4  ...[more]

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