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Identification of the molecular targets of miR-185 in DN3 stage thymocytes

ABSTRACT: T cell-specific overexpression of miR-185 caused a developmental block at DN3 stage of thymopoiesis. DN3 stage thymocytes were sorted from the wild type (C57BL/6) and miR-185 Tg mice, followed by total RNA isolation.

ORGANISM(S): Mus musculus

SUBMITTER: Nicolai S.C. van Oers

PROVIDER: E-GEOD-49057 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transgenic expression of microRNA-185 causes a developmental arrest of T cells by targeting multiple genes including Mzb1.

Belkaya Serkan S Murray Sean E SE Eitson Jennifer L JL de la Morena M Teresa MT Forman James A JA van Oers Nicolai S C NSC

The Journal of biological chemistry 20130906 42

miR-185 is a microRNA (miR) that targets Bruton's tyrosine kinase in B cells, with reductions in miR-185 linked to B cell autoantibody production. In hippocampal neurons, miR-185 targets both sarcoplasmic/endoplasmic reticulum calcium ATPase 2 and a novel Golgi inhibitor. This miR is haploinsufficient in 90-95% of individuals with chromosome 22q11.2 deletion syndrome, patients who can present with immune, cardiac, and parathyroid problems, learning disorders, and a high incidence of schizophreni ...[more]

PMID: 24014023

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