Unknown,Transcriptomics,Genomics,Proteomics

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INO80 complex in the core regulatory network governing ESC self-renewal [ChIP-Seq]


ABSTRACT: Embryonic stem cell (ESC) self-renewal and pluripotency is controlled by the coordinated action of transcription factors and chromatin regulators. Compared to the pluripotency transcription factors, the function of the chromatin regulators, especially the ATP-dependent chromatin remodelers, remains poorly understood in ESCs. Here, we show that INO80, a SWI/SNF family chromatin remodeling complex, is essential for ESC self-renewal, pluripotency, somatic cell reprogramming, and embryonic development. Ino80, the ATPase of the complex, forms an auto-regulatory loop with the ESC master transcription factors Oct4, Nanog, and Sox2. More importantly, it co-occupies the enhancer regions of most key pluripotency genes with the master transcription factors, and positively regulates their expression by maintaining an open chromatin structure. Our data suggests that INO80 is an integral component of the pluripotency transcription network, and plays a critical role in both the maintenance and establishment of pluripotency Identification of Ino80 localization in mouse embryonic stem cells

ORGANISM(S): Mus musculus

SUBMITTER: Li Wang 

PROVIDER: E-GEOD-49137 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

INO80 facilitates pluripotency gene activation in embryonic stem cell self-renewal, reprogramming, and blastocyst development.

Wang Li L   Du Ying Y   Ward James M JM   Shimbo Takashi T   Lackford Brad B   Zheng Xiaofeng X   Miao Yi-liang YL   Zhou Bingying B   Han Leng L   Fargo David C DC   Jothi Raja R   Williams Carmen J CJ   Wade Paul A PA   Hu Guang G  

Cell stem cell 20140501 5


The master transcription factors play integral roles in the pluripotency transcription circuitry of embryonic stem cells (ESCs). How they selectively activate expression of the pluripotency network while simultaneously repressing genes involved in differentiation is not fully understood. Here, we define a requirement for the INO80 complex, a SWI/SNF family chromatin remodeler, in ESC self-renewal, somatic cell reprogramming, and blastocyst development. We show that Ino80, the chromatin remodelin  ...[more]

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