Proteomics

Dataset Information

0

A mass spectrometry survey of chromatin-associated proteins in pluripotency and early lineage commitment

ABSTRACT: Pluripotent Embryonic Stem Cells (ESCs) can be captured in vitro in different states, ranging from unrestricted ‘naïve’ to more developmentally constrained ‘primed’ pluripotency. Complexes involved in epigenetic regulation and key transcription factors have been shown to be involved in specifying these distinct states. In this study, we use proteomic profiling of the chromatin landscape in naive pluripotent ESCs, Epistem cells (EpiSCs) and early differentiated ESCs to survey the chromatin in naïve and primed pluripotency and during differentiation. We provide a comprehensive overview of epigenetic complexes situated on the chromatin and identify proteins associated with the maintenance and loss of pluripotency. The findings presented here indicate major compositional alterations of epigenetic complexes starting from ESC priming onwards. Our results contribute to the understanding of ESC differentiation and provide a framework for future studies of lineage commitment of ESCs.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Guido van Mierlo  

LAB HEAD: Hendrik Marks

PROVIDER: PXD011782 | Pride | 2019-07-04

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
pride_submission.rar Other
proteinGroupsChromatin.txt Txt
Items per page:
1 - 2 of 2
altmetric image

Publications

A Mass Spectrometry Survey of Chromatin-Associated Proteins in Pluripotency and Early Lineage Commitment.

van Mierlo Guido G   Wester Roelof Alexander RA   Marks Hendrik H  

Proteomics 20190703 14

Pluripotency can be captured in vitro in the form of Embryonic Stem Cells (ESCs). These ESCs can be either maintained in the unrestricted "naïve" state of pluripotency, adapted to developmentally more constrained "primed" pluripotency or differentiated towards each of the three germ layers. Epigenetic protein complexes and transcription factors have been shown to specify and instruct transitions from ESCs to distinct cell states. In this study, proteomic profiling of the chromatin landscape by c  ...[more]

Similar Datasets

Proteomics OCT4 IP-MS in Naive and Primed Human ESCs
2021-09-12 | PXD026556 | Pride
Genomics HP1β has distinct subnuclear localizations, chromatin binding features and functions in embryonic stem cells and differentiating cells [ChIP-seq]
2015-08-02 | E-GEOD-64946 | biostudies-arrayexpress
Transcriptomics An Intermediate Pluripotent State Controlled by microRNAs is Required for the Naïve to Primed Stem Cell Transition
2018-03-29 | GSE112334 | GEO
Metabolomics Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells (hPSCs)
2020-05-08 | ST001377 | MetabolomicsWorkbench
Proteomics Integrative proteomics reveals a protective role of PRC2 for the ground state pluripotent epigenome
2018-11-21 | PXD007154 | Pride
Proteomics A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naïve pluripotency in mouse embryonic stem cells
2018-10-18 | PXD010670 | Pride
Transcriptomics m6A RNA Methylation is Critical for Adequate Exit from Naïve Pluripotency and Execution of Mammalian Development (RNA-Seq)
2014-11-02 | E-GEOD-61997 | biostudies-arrayexpress
Genomics Polycomb Group Protein Pcgf6 Acts as a Master Regulator to Maintain Embryonic Stem Cell Identity
2016-07-28 | E-GEOD-84905 | biostudies-arrayexpress
Proteomics The translational landscape of naïve and primed state pluripotency
2020-04-03 | PXD014528 | Pride
Transcriptomics Dazl is required for TET-mediated reprogramming to a naïve pluripotent state (II)
2015-07-01 | E-GEOD-69356 | biostudies-arrayexpress