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Transcription profiling of early response of Arabidopsis root tips exposed to 1 hour of red light

ABSTRACT: Red light can affect a variety of responses in Arabidopsis. We characterize the early gene expression patterns of roots exposed to 1 hour of red light. Early genes indicate elements involved in photomorphogenesis, chloroplast development, PAL pathways, root hair development are regulated by 1 hour of red light; We used microarrays to detail the gene expression underlying the effects of red light on roots. Experiment Overall Design: 7 Day dark-grown 1 cm Arabidopsis root tips were harvested for RNA extraction and hybridization on Affymetrix microarrays. We pooled samples from several Petri plates for each experiment to obtain enough RNA for arrays. Three experiments were performed. Treatment 1 hour of red light 630 nm (max) and dark controls

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Melanie Correll

PROVIDER: E-GEOD-4933 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene profiling of the red light signalling pathways in roots.

Molas Maria Lia ML Kiss John Z JZ Correll Melanie J MJ

Journal of experimental botany 20060814 12

Red light, acting through the phytochromes, controls numerous aspects of plant development. Many of the signal transduction elements downstream of the phytochromes have been identified in the aerial portions of the plant; however, very few elements in red-light signalling have been identified specifically for roots. Gene profiling studies using microarrays and quantitative Real-Time PCR were performed to characterize gene expression changes in roots of Arabidopsis seedlings exposed to 1 h of red ...[more]

PMID: 16908503

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