Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis ahg1 and ahg3 mutants


ABSTRACT: The effect of ahg1 and ahg3 on the gene expression profiles is similar but some genes are differentially affected. Experiment Overall Design: To investigate the effect of ahg1 and ahg3 mutations, the genome wide gene expression profiles of germinating seedlings were examined. These mutations occurred in close but distinct PP2C genes. Stratified seeds were transferred to plates containing 0.5 uM ABA and allowed germinate at 23 °C under 16hr light 8hr dark conditions. After two days samples were collected for RNA extraction.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Takashi Hirayama 

PROVIDER: E-GEOD-6638 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed.

Nishimura Noriyuki N   Yoshida Tomo T   Kitahata Nobutaka N   Asami Tadao T   Shinozaki Kazuo K   Hirayama Takashi T  

The Plant journal : for cell and molecular biology 20070425 6


The phytohormone abscisic acid (ABA) regulates physiologically important stress and developmental responses in plants. To reveal the mechanism of response to ABA, we isolated several novel ABA-hypersensitive Arabidopsis thaliana mutants, named ahg (ABA-hypersensitive germination). ahg1-1 mutants showed hypersensitivity to ABA, NaCl, KCl, mannitol, glucose and sucrose during germination and post-germination growth, but did not display any significant phenotypes in adult plants. ahg1-1 seeds accum  ...[more]

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