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Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of HDAC3-depleted mouse liver rescued with wild-type exogenous HDAC3

ABSTRACT: We have shown that intravenous injection of HDAC3 floxed mice with adeno-associated virus (AAV) expressing Cre depletes hepatic HDAC3, upregulates lipogenic gene expression, and causes fatty liver. When AAV-Flag-HDAC3 wild-type (WT) is co-injected along with AAV-Cre, the exogenous HDAC3 is expressed at endogenous levels and can completely rescue fatty liver phenotype. Here we profile transcriptome of the rescued WT livers in comparison with HDAC3-depleted (KO) livers. 4-months old C57BL/6 male mice were co-injected with AAV-Cre or AAV-Cre plus AAV-Flag-HDAC3. Mice were fed ad libitum and harvested at 5 pm (ZT10) at 2-weeks post-injection. Liver total RNA was extracted and hybridized to Affymetrix Mouse Gene 1.0ST array.

ORGANISM(S): Mus musculus

SUBMITTER: Zheng Sun

PROVIDER: E-GEOD-49386 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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