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Development of gene expression signatures for LGR5hi and LGR5lo ISCs in response to gamma-irradiation

ABSTRACT: To check gene expression signatures for LGR5hi and LGR5lo ISCs in response to gamma-irradiation, we performed whole genome microarray on LGR5hi and LGR5lo ISCs purified from irradiated mice or non-irradiated mice Radiation induced gene expression in LGR5hi and LGR5lo ISCs of LGR5-GFPki mice was measured at 3 hours after exposure to 12Gy γ-irradiation or non-irradiation.

ORGANISM(S): Mus musculus

SUBMITTER: Hans Kestler

PROVIDER: E-GEOD-49461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Development of gene expression signatures for LGR5hi and LGR5lo ISCs in response to gamma-irradiation

Project description:To check gene expression signatures for LGR5hi and LGR5lo ISCs in response to gamma-irradiation, we performed whole genome microarray on LGR5hi and LGR5lo ISCs purified from irradiated mice or non-irradiated mice

2013-08-01 | GSE49461 | GEO

Role of polycomb repressive complex 1 in the intestinal homeostasis [ChIP-Seq]

Project description:These data include the genome wide occupancy of histone modifications and transcription factors by ChIP sequencing in mouse crypt cells and in mouse ISCs. Immuno-precipitation of formaldehyde cross-linked chromatin prepared from wild type and Ring1a Ring1b dKO crypt cells and from WT ISCs using specific antibody against different target protein/modification.

2015-10-29 | E-GEOD-65318 | biostudies-arrayexpress

Role of polycomb repressive complex 1 in the intestinal homeostasis [RNA-Seq]

Project description:These data include RNA Seq data generated from wild type and Ring1a Ring1b dKO ISCs from Lgr5Gfp-CreERT2, Lgr5Gfp-CreERT2 Ring1a-/- Ring1bf/f, Lgr5Gfp-CreERT2-Ctnnb1-ex3 and Lgr5Gfp-CreERT2 Ring1a-/- Ring1bf/f-Ctnnb1-ex3 mice. Total RNA extracted from sorted wild type and Ring1a Ring1b dKO ISCs.

2015-10-29 | E-GEOD-65321 | biostudies-arrayexpress

Role of polycomb repressive complex 1 in the intestinal homeostasis [ChIP-seq 2]

Project description:These data include the genome wide occupancy of histone modifications and transcription factors by ChIP sequencing in mouse villi cells and in mouse ISCs. Immuno-precipitation of formaldehyde cross-linked chromatin prepared from wild type Villi cells and wild type and Ring1a Ring1b dKO ISCs using specific antibody against different target protein/modification.

2015-10-29 | E-GEOD-71946 | biostudies-arrayexpress

RNA-seq of mouse Lgr5+ intestinal stem cells and progenitors following Lkb1 deletion against controls

Project description:Perturbed intestinal epithelial homeostasis demonstrated as decreased Lgr5+ intestinal stem cells (Lgr5 ISCs) and increased secretory lineages were observed in our study where Lkb1 was specfically deleted in Lgr5 ISCs using Lgr5-EGFP-creERT2 (Tamoxifen) deletor. To gain mechanistic insight how Lkb1 maintains intestinal epithelial stem cell homeostasis, Lkb1 deficient ISCs (Lgr5-high cells) and progenitors (Lgr5-low cells) are isolated by flow cytometry and profiled by RNA sequencing to compare with controls (Lkb1 wild type ISCs and progenitors).

2020-02-29 | E-MTAB-6501 | biostudies-arrayexpress

Mus musculus

Project description:Development of gene expression signatures for LGR5hi and LGR5lo ISCs in response to gamma-irradiation

| PRJNA213968 | ENA

Role of polycomb repressive complex 1 in the intestinal homeostasis

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-10-29 | E-GEOD-65322 | biostudies-arrayexpress

Expression data of wild type, dDP mutant, and de2f1, deleted in the posterior, Drosophila 3rd instar larval eye imaginal discs

Project description:Apoptosis is an important process to eliminate cells from tissue which have incurred irreparable DNA damage. While dE2F1/dDP complexes respond to such damage by transcriptionally activating apoptotic genes, previous data suggests that activation of the previously characterized apoptotic target genes of dE2F1/dDP alone may not be the only gene regulation important for gamma irradiation-induced apoptosis. Here we report that following irradiation in dDP mutant 3rd instar larval eye imaginal discs, many genes important for oxidative phosphorylation are down-regulated, which are not down-regulated following irradiation in wild type eye discs. Biological triplicates of wild type, dDP mutant and de2f1, deleted in the posterior, eye discs were untreated or irradiated with 40Gy of gamma radiation. Total RNA was extracted by Trizol from untreated eye discs and from irradiated eye discs 4h after irradiation. RNA was column purified. PCR amplified RNAs were hybridized on Affymetrix Drosophila Genome 2.0 Array.

2013-11-25 | E-GEOD-39389 | biostudies-arrayexpress

Transcription profiling of Drosophila Kc167 cells treaye with ecdysone and gamma irradiation to investigate ecdysone- and stress-induced expression profiles

Project description:Kc167 cells were mock-treated/treated with combinations of steroid hormone ecdysone and gamma-irradiation, and harvested. The expression profiles were determined using Affymetrix Drosophila Genechip 1 arrays. Comparisons between the sample groups allow the identification of genes with ecdysone- and/or radiation-responsive expression patterns. Experiment Overall Design: 1 set of control, ecdysone, gamma-irradiated, ecdysone + irradiated samples was analyzed.

2008-06-12 | E-GEOD-3072 | biostudies-arrayexpress

Response of mammary tissue to high-LET HZE particle (Silicon ions) radiation or low-LET gamma-rays

Project description:Transcriptional profiling of mammary tissue irradiated at 10 weeks of age with either 100 cGy sparsely ionizing gamma-rays, or 10 cGy or 30 cGy densely ionizing radiation (350 MeV/amu Si). Mammary tissue was collected 1 weeks, 4 weeks, and 12 weeks post-irradiation. Four radiation treatment groups: sham, 100 cGy sparsely ionizing gamma-rays, 10 cGy or 30 cGy densely ionizing radiation (350 MeV/amu Si). Three time points post-irradiation (1, 4, and 12 weeks). Three or four replicates per time point.

2013-11-30 | E-GEOD-48392 | biostudies-arrayexpress

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