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Comparison of gene expression in embryos in wich Smicl has been knocked-down and in control embryos

ABSTRACT: Embryos were injected with either control or Smicl antisense morpholino oligonucleotides and harvested at Stage 10.5 for RNA analysis. Keywords: Knock-down analysis Embryos from three different spawnings where injected at one cell stage with either control or Smicl antisense morpholino oligonucleotides and cultured to early gastrula stage 10.5 for RNA isolation. Some embryos were cultured to further development to check their phenotypes. Each slide was hybridised with a mixture 1:1 of sample and control cDNAs, each labelled with a different dye. Hybridisations were carried out as dye-swapped technical replicates, therefore six microarray slides were hybridized

ORGANISM(S): Xenopus laevis

SUBMITTER: Jim Smith 

PROVIDER: E-GEOD-4952 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Smicl is required for phosphorylation of RNA polymerase II and affects 3'-end processing of RNA at the midblastula transition in Xenopus.

Collart Clara C   Ramis Joana M JM   Down Thomas A TA   Smith James C JC  

Development (Cambridge, England) 20091001 20

Smicl (Smad-interacting CPSF 30-like) is an unusual protein that interacts with transcription factors as well as with the cleavage and polyadenylation specificity factor (CPSF). Previous work has shown that Smicl is expressed maternally in the Xenopus embryo and is later required for transcription of Chordin. In this paper we search for additional targets of Smicl. We identify many genes whose onset of expression at the midblastula transition (MBT) requires Smicl and is correlated with the trans  ...[more]

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