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Transcription profiling of Xenopus laevis early gastrulation embryos injected with antisense oligonucleotides against TBP, TLF or TBP2

ABSTRACT: Xenopus laevis embryos were injected with antisense oligonucleotides against TBP, TLF or TBP2. Embryos were allowed to develop up to stage 7 (non-injected control embryos) or stage 10.5 (early gastrula, control and antisense injected embryos) and subsequently collected for RNA and protein isolation. The transcriptome profile of antisense injected and control embryos was compared.

ORGANISM(S): Xenopus laevis

SUBMITTER: Ulrike Jacobi

PROVIDER: E-MEXP-1009 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TBP paralogs accommodate metazoan- and vertebrate-specific developmental gene regulation.

Jacobi Ulrike G UG Akkers Robert C RC Pierson Elisabeth S ES Weeks Daniel L DL Dagle John M JM Veenstra Gert Jan C GJ

The EMBO journal 20070816 17

In addition to TATA-binding protein (TBP), a key factor for transcription initiation, the metazoan-specific TBP-like factor TLF/TRF2 and the vertebrate-specific factor TBP2/TRF3 are known to be required for transcription of specific subsets of genes. We have combined an antisense-knockdown approach with transcriptome profiling to determine the significance and biological role of TBP-independent transcription in early gastrula-stage Xenopus laevis embryos. Here, we report that, although each of t ...[more]

PMID: 17703192

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