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Stretch responsive miRNAs in human aortic valve interstitial cells

ABSTRACT: miRNA-Sequencing was performed on human aortic valve interestitial cells (AVICs) exposed to 14% stretch at 1 hz or static conditions for 24h. Six static control and six samples exposed to cyclic stretch 14% for 24h

ORGANISM(S): Homo sapiens

SUBMITTER: Vishal Nigam

PROVIDER: E-GEOD-49558 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The stretch responsive microRNA miR-148a-3p is a novel repressor of IKBKB, NF-κB signaling, and inflammatory gene expression in human aortic valve cells.

Patel Vishal V Carrion Katrina K Hollands Andrew A Hinton Andrew A Gallegos Thomas T Dyo Jeffrey J Sasik Roman R Leire Emma E Hardiman Gary G Mohamed Salah A SA Nigam Sanjay S King Charles C CC Nizet Victor V Nigam Vishal V

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20150128 5

Bicuspid aortic valves calcify at a significantly higher rate than normal aortic valves, a process that involves increased inflammation. Because we have previously found that bicuspid aortic valve experience greater stretch, we investigated the potential connection between stretch and inflammation in human aortic valve interstitial cells (AVICs). Microarray, quantitative PCR (qPCR), and protein assays performed on AVICs exposed to cyclic stretch showed that stretch was sufficient to increase exp ...[more]

PMID: 25630970

Similar Datasets

Stretch responsive miRNAs in human aortic valve interstitial cells

Project description:miRNA-Sequencing was performed on human aortic valve interestitial cells (AVICs) exposed to 14% stretch at 1 hz or static conditions for 24h.

2015-01-09 | GSE49558 | GEO

Comparison of human aortic valve interstitial cells (AVICs) exposed to cyclic stretch to static samples

Project description:AVICs were exposed to cyclic stretch to examine the role of mechanical stimuli on gene expression AVICs cultured on collagen 1 coated Bioflex were exposed to 14% stretch at 1 hz or static conditions using a Flexcell-5000 14% stretch was the experimental condition while the static condition was the control

2015-01-09 | E-GEOD-47687 | biostudies-arrayexpress

Comparison of human aortic valve interstitial cells (AVICs) exposed to cyclic stretch to static samples

2015-01-09 | GSE47687 | GEO

Identification of Stretch Responsive microRNAs in Embyonic Mouse Cardiomyocytes

Project description:The goal of this study was to identify microRNAs that are modulated by cyclic stretch. miRNA-Seq was performed comparing samples from cultured E16.5 mouse cardiomyocytes exposed to either cyclic stretch of 16% at 1Hz for 24h or static conditions.

2020-01-22 | GSE120676 | GEO

The long non-coding HOTAIR is modulated by cyclic stretch and WNT/beta-CATENIN in human aortic valve cells and is a novel repressor of calcification genes

Project description:Aortic valve calcification is a significant and serious clinical problem for which there are no effective medical treatments. Individuals born with bicuspid aortic valves, 1-2% of the population, are at the highest risk of developing aortic valve calcification. Aortic valve calcification involves increased levels of calcification and inflammatory genes. Bicuspid aortic valve leaflets experience increased strain. The molecular mechanisms involved in the pathogenesis of calcification of BAVs are not well understood, especially the molecular response to mechanical stretch. HOTAIR is a long non-coding RNA (lncRNA) that has been implicated with cancer but has not been studied in cardiac disease. We have found that HOTAIR levels are decreased in BAVs and in human aortic interstitial cells (AVICs) exposed to cyclic stretch. Reducing HOTAIR levels via siRNA in AVICs results in increased expression of calcification genes.

2014-05-02 | E-MTAB-2409 | biostudies-arrayexpress

Changes in B10F10 melanoma cells in response to stretch

Project description:To study the effects induced by mechanical stretch on cancer cells, B16F10 murine melanoma cells were seeded in Flexcell plates and cultured in static or stretch (15% equibiaxial elongation, 15 cycles/min) for 24 hours. Cells were collected, and RNA isolated and sequenced.

2022-02-14 | GSE168499 | GEO

Stretch-dependent genes in vascular smooth muscle cells

Project description:Vascular smooth muscle cells (VSMCs) respond to biomechanical stretch with specific changes in gene expression which govern the phenotype of these cells. The mechanotransducer zyxin is a potential candidate for regulating the expression of such genes. Using microarrays, we compared stretch-induced gene expression in wild type and zyxin-null VSMCs to define such changes in detail. Wild type (WT) and zyxin-null VSMCs were stretched at 10% cyclic elongation for 6 hours and the changes in gene expression were compared under static and stretched conditions. Up to 3 biological replicates were used for each of the 4 sample types.

2015-07-01 | E-GEOD-60447 | biostudies-arrayexpress

Homo sapiens

Project description:Comparison of human aortic valve interstitial cells (AVICs) exposed to cyclic stretch to static samples

| PRJNA207296 | ENA

Transcription profiling of rat organotypic hippocampal cultures following mild or severe stretch injury

Project description:Microarray study of gene expression and molecular interaction pathways in an in vitro model of graded diffuse axonal injury. <br>Mild (10%) and severe (50%) stretch injuries were delivered to rats organotypic hippocampal cultures and mRNA levels were analyzed at 24h. Despite the lack of significant cell death, there was more activation of complex cellular mechanisms following 10% stretch than 50%. IL-1? played a central role in molecular interactions of both injury groups but additional pathways of neurodegeneration involving RhoA were found in 50% stretch.<br><br>

2009-07-04 | E-MEXP-2235 | biostudies-arrayexpress

Changes in transcription profile in pelvic organ fibroblasts in response to stretch

Project description:Failure of ligamentous support of the genital tract to resist intra-abdominal pressure is a plausible underlying mechanism for the development of pelvic organ prolapse, but the nature of molecular response of pelvic tissue support remains unknown. We hypothesized that the expression of genes coding for proteins involved in maintaining the cellular and extracellular integrity would be altered in cases of pelvic organ prolapse. Therefore, cDNA microarrays were used to examine the difference in transcriptional profile in RNA of primary culture fibroblasts subjected to mechanical stretch and those that remained static. Keywords: Changes in transcription profile in pelvic organ fibroblasts in response to stretch

2007-01-26 | GSE6862 | GEO

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