Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

In-culture titration experiment for sRSE

ABSTRACT: In order to test the transcriptome-wide functionality of the identified sRSE instances, we performed an in vivo titration experiment in which synthetic RNA oligonucleotides harboring tandem sRSE1 repeats were used as intracellular decoys that would bind the putative trans factor, preventing it from targeting endogenous transcripts. Capped and poly-adenylated RNAs carrying tandem sRSE elements were transfected along with scrambled RNA molecules as control. 48 hours post-transfection, samples were subjected to transcriptome profiling to measure the regulatory consequences of the in-vivo titration of the sRSE-binding trans factor.

ORGANISM(S): Homo sapiens

SUBMITTER: Hani Goodarzi 

PROVIDER: E-GEOD-49609 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Metastasis-suppressor transcript destabilization through TARBP2 binding of mRNA hairpins.

Goodarzi Hani H   Zhang Steven S   Buss Colin G CG   Fish Lisa L   Tavazoie Saeed S   Tavazoie Sohail F SF  

Nature 20140709 7517

Aberrant regulation of RNA stability has an important role in many disease states. Deregulated post-transcriptional modulation, such as that governed by microRNAs targeting linear sequence elements in messenger RNAs, has been implicated in the progression of many cancer types. A defining feature of RNA is its ability to fold into structures. However, the roles of structural mRNA elements in cancer progression remain unexplored. Here we performed an unbiased search for post-transcriptional modula  ...[more]

Similar Datasets

Transcriptomics In-culture titration experiment for sRSE
2014-07-24 | GSE49609 | GEO
Genomics Homo sapiens
| PRJNA214607 | ENA
Transcriptomics Metastasis-suppressor transcript destabilization through TARBP2 binding of mRNA hairpins
2014-07-16 | E-GEOD-49649 | biostudies-arrayexpress
Transcriptomics sRSM1 synthetic decoy vs. scrambled transfections in MDA-MB-231 cells
2012-02-28 | E-GEOD-35749 | biostudies-arrayexpress
Transcriptomics Broad-Spectrum Therapeutic Suppression of Metastatic Melanoma Through Nuclear Hormone Receptor Activation
2014-06-01 | E-GEOD-48782 | biostudies-arrayexpress
Transcriptomics Regulatory consequences of SOX4 knock-down in MDA-LM2 cell line
2016-03-15 | E-GEOD-79202 | biostudies-arrayexpress
Transcriptomics Regulatory consequences of TARBP2 knock-down in Dicer-null MEFs
2014-07-24 | E-GEOD-56949 | biostudies-arrayexpress
Transcriptomics Regulatory consequences of TARBP2 knock-down in293T cell-line
2014-07-24 | E-GEOD-56945 | biostudies-arrayexpress
Transcriptomics Regulatory consequences of TARBP2 knock-down in CN-LM1a cell-line
2014-07-24 | E-GEOD-56947 | biostudies-arrayexpress
Transcriptomics Regulatory consequences of TARBP2 knock-down in MDA-LM2 cell-line
2014-07-24 | E-GEOD-49611 | biostudies-arrayexpress