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Differential RNA-sequencing of Campylobacter jejuni fur perR mutant

ABSTRACT: We report the use of differential RNA-sequencing for the determination of the primary transcriptome of the fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites. Campylobacter jejuni NCTC 11168 fur perR mutant was grown to late log phase, RNA was purified and used for differential RNA-sequencing by 454 sequencing with barcoded libraries, and used for determination of genome-wide transcription start sites

ORGANISM(S): Campylobacter jejuni

SUBMITTER: Arnoud van Vliet

PROVIDER: E-GEOD-49660 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

Project description:We report the use of RNA-seq analysis for the determination of RPKM transcript levels in wildtype and fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for comparison of gene expression levels. Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant were grown to late log phase, RNA was purified and used for RNA-sequencing by Illumina HiSeq sequencing

2014-07-01 | E-GEOD-49687 | biostudies-arrayexpress

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