Dataset Information

Global functional impacts of Campylobacter jejuni stress induced by exposure to deoxycholate

ABSTRACT: Comparison of the Campylobacter jejuni NCTC 11168 proteome in MH media in the presence and absence of Deoxycholate

OTHER RELATED OMICS DATASETS IN: GSE89641PRJNA352929

INSTRUMENT(S): Q Exactive

ORGANISM(S): Campylobacter Jejuni

SUBMITTER: Matthew Monroe

LAB HEAD: Joshua Adkins

PROVIDER: PXD005306 | Pride | 2017-11-21

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Cj_media_DOC_R1_23Feb15_Arwen_14-12-03.mgf	Mgf
	Cj_media_DOC_R1_23Feb15_Arwen_14-12-03.pride.mgf.gz	Mgf
	Cj_media_DOC_R1_23Feb15_Arwen_14-12-03.raw	Raw
	Cj_media_DOC_R1_23Feb15_Arwen_14-12-03_msgfplus.mzid.gz	Mzid
	Cj_media_DOC_R2_23Feb15_Arwen_14-12-03.mgf	Mgf

Items per page:

1 - 5 of 32

Publications

The food-borne pathogen Campylobacter jejuni responds to the bile salt deoxycholate with countermeasures to reactive oxygen species.

Negretti Nicholas M NM Gourley Christopher R CR Clair Geremy G Adkins Joshua N JN Konkel Michael E ME

Scientific reports 20171113 1

Bile plays an important role in digestion, absorption of fats, and the excretion of waste products, while concurrently providing a critical barrier against colonization by harmful bacteria. Previous studies have demonstrated that gut pathogens react to bile by adapting their protein synthesis. The ability of pathogens to respond to bile is remarkably complex and still incompletely understood. Here we show that Campylobacter jejuni, a leading bacterial cause of human diarrheal illness worldwide, ...[more]

PMID: 29133896

Similar Datasets

Project description:C. jejuni, a spiral-shaped gram-negative bacterium, is a leading bacterial cause of human foodborne illness. Acute disease is associated with C. jejuni invasion of the intestinal epithelium. Further, maximal host cell invasion requires the secretion of proteins termed Campylobacter invasion antigens (Cia). As bile acids are known to alter the pathogenic behavior of other gastrointestinal pathogens, we hypothesized that the virulence potential of Campylobacter may be triggered by the bile acid deoxycholate (DOC). In support of this hypothesis, culturing C. jejuni with a physiologically relevant concentration of DOC significantly altered the kinetics of cell invasion as evidenced by gentamicin-protection assays. In contrast to C. jejuni harvested from Mueller-Hinton (MH) agar plates, C. jejuni harvested from MH agar plates supplemented with DOC demonstrated Cia secretion as judged by metabolic labeling experiments. DOC was also found to induce the expression of the ciaB gene as judged by B-galactosidase reporter assays and real-time RT-PCR. Microarray analysis revealed that DOC induced the expression of virulence genes (i.e., ciaB, cmeABC, dccR, and tlyA). In summary, we demonstrate that it is possible to enhance the pathogenic behavior of C. jejuni by modifying the culture conditions. These results provide a foundation to identify genes expressed by C. jejuni in response to in vivo-like culture conditions. Keywords: Stress response For the expression profiling arrays, an indirect comparison of gene expression was performed, where the expression profile of the C. jejuni F38011 cultured in the presence and absence of DOC was measured separately on different slides as described previously (26). Briefly, Cy5 labeled reference DNA from the C. jejuni F38011 strain was mixed with Cy3 labeled test cDNA (C. jejuni F38011 cultured in the presence or absence of DOC) and hybridized to the Campylobacter cDNA array (26) on separate slides. DNA microarrays were scanned using an Axon GenePix 4000B microarray laser scanner (Axon Instruments, Union City, CA) and the data for spot and background intensities were processed using the GenePix 4.0 software. To compensate for any effect of the amount of template and uneven Cy-dye incorporation, data normalization was performed as previously described (26). For the comparison of genes differentially expressed in the presence and absence of DOC,six hybridization measurements were generated per biological experiment (three technical replicate arrays and two replicate features per array).