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Next Generation Metatranscriptome Sequencing of an Anaerobic Hexadecane-Degrading Microbial Consortia

ABSTRACT: Purpose: Identification of transcriptionally active genes in the unculturable community constituent, Smithella, during hexadecane degradation; Differential gene expression analysis of hexadecane-relevant genes acoss three different conditions; Extension of metatranscriptomic datasets to other community constituents to identify interspecies relationships. mRNA profiles were generated for this community across three different conditions (hexadecane-, butyric acid-, caprylic acid-degrading conditions) using a modified version of Nextera and sequenced using Illumina's Miseq platform.

ORGANISM(S): mixed culture KAZ_0815

SUBMITTER: Mallory Embree

PROVIDER: E-GEOD-49830 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-cell genome and metatranscriptome sequencing reveal metabolic interactions of an alkane-degrading methanogenic community.

Embree Mallory M Nagarajan Harish H Movahedi Narjes N Chitsaz Hamidreza H Zengler Karsten K

The ISME journal 20131024 4

Microbial interactions have a key role in global geochemical cycles. Although we possess significant knowledge about the general biochemical processes occurring in microbial communities, we are often unable to decipher key functions of individual microorganisms within the environment in part owing to the inability to cultivate or study them in isolation. Here, we circumvent this shortcoming through the use of single-cell genome sequencing and a novel low-input metatranscriptomics protocol to rev ...[more]

PMID: 24152715

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