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ChIP-seq analysis of M1BP in Drosophila S2R+ cells

ABSTRACT: The genomic distribution of a novel transcription factor called M1BP was determined in Drosophila S2R+ cells Polyclonal antibody raised against M1BP was used to immunoprecipitate M1BP-DNA adducts generated by treating Drosophila cells with formaldehyde, lysing the cells, and shearing DNA by sonication. Immunoprecipitated DNA was sequenced using the AB SOLiD system.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: David Gilmour

PROVIDER: E-GEOD-49842 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Distinct mechanisms of transcriptional pausing orchestrated by GAGA factor and M1BP, a novel transcription factor.

Li Jian J Gilmour David S DS

The EMBO journal 20130524 13

Thousands of genes in Drosophila have Pol II paused in the promoter proximal region. Almost half of these genes are associated with either GAGA factor (GAF) or a newly discovered factor we call M1BP. Although both factors dictate the association of Pol II at their target promoters, they are nearly mutually exclusive on the genome and mediate different mechanisms of regulation. High-resolution mapping of Pol II using permanganate-ChIP-seq indicates that pausing on M1BP genes is transient and coul ...[more]

PMID: 23708796

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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