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High-throughput sequencing of Drosophila melanogaster small RNAs from S2R+ cells

ABSTRACT: High-throughput sequencing of Drosophila melanogaster small RNAs from S2R+ cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster S2R+ cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Nicolas Robine

PROVIDER: E-GEOD-13679 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dicing of viral replication intermediates during silencing of latent Drosophila viruses.

Flynt Alex A Liu Na N Martin Raquel R Lai Eric C EC

Proceedings of the National Academy of Sciences of the United States of America 20090227 13

Previous studies revealed roles for RNA interference (RNAi) in the immediate cellular response to viral infection in plants, nematodes and flies. However, little is known about how RNAi combats viruses during persistent or latent infections. Our analysis of small RNAs cloned from Drosophila cells latently infected with Flock House Virus (FHV) failed to reveal signatures of bulk degradation of the viral genome. Instead, this + strand virus specifically generated Dicer-2-dependent, 21-nucleotide s ...[more]

PMID: 19251644

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