Unknown,Transcriptomics,Genomics,Proteomics

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SND1 Transcription Factor-Directed Quantitative Functional Hierarchical Genetic Regulatory Network in Wood Formation in Populus trichocarpa


ABSTRACT: We focused on RNA-seq-based full transcriptome responses to PtrSND1-B1 overexpression at 7, 12, and 25 h in stem defferentiating xylem (SDX) protoplasts We transfected PtrSND1-B1 and sGFP into stem differentiating xylem protoplasts and performed RNA-seq to reveal the whole transcriptome.

ORGANISM(S): Populus trichocarpa

SUBMITTER: Ying-Chung Lin 

PROVIDER: E-GEOD-49911 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

SND1 transcription factor-directed quantitative functional hierarchical genetic regulatory network in wood formation in Populus trichocarpa.

Lin Ying-Chung YC   Li Wei W   Sun Ying-Hsuan YH   Kumari Sapna S   Wei Hairong H   Li Quanzi Q   Tunlaya-Anukit Sermsawat S   Sederoff Ronald R RR   Chiang Vincent L VL  

The Plant cell 20131126 11


Wood is an essential renewable raw material for industrial products and energy. However, knowledge of the genetic regulation of wood formation is limited. We developed a genome-wide high-throughput system for the discovery and validation of specific transcription factor (TF)-directed hierarchical gene regulatory networks (hGRNs) in wood formation. This system depends on a new robust procedure for isolation and transfection of Populus trichocarpa stem differentiating xylem protoplasts. We overexp  ...[more]

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