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Genes regulated by HS3ST2

ABSTRACT: We used Affymetrix HG U133 Plus 2.0 GeneChips to compare the transcriptome of HS3ST2-transfected and control vector-transfected MDA-MB-231 cells. MDA-MB-231 cells were transfected with HS3ST2- or control-vector. Subsequently, total RNA was collected and processed for analysis using Affymetrix microarrays. Three independent replicates were prepared for each comparison group.

ORGANISM(S): Homo sapiens

SUBMITTER: George Yip

PROVIDER: E-GEOD-49962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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HS3ST2 modulates breast cancer cell invasiveness via MAP kinase- and Tcf4 (Tcf7l2)-dependent regulation of protease and cadherin expression.

Vijaya Kumar Archana A Salem Gassar Ezeddin E Spillmann Dorothe D Stock Christian C Sen Yin-Ping YP Zhang Ting T Van Kuppevelt Toin H TH Hülsewig Carolin C Koszlowski Eliene O EO Pavao Mauro S G MS Ibrahim Sherif A SA Poeter Michaela M Rescher Ursula U Kiesel Ludwig L Koduru Suresh S Yip George W GW Götte Martin M

International journal of cancer 20140430 11

Heparan sulfate 3-O-sulfotransferase 2 (HS3ST2), an enzyme mediating 3-O-sulfation of heparan sulfate (HS), is silenced by hypermethylation in breast cancer. As HS has an important co-receptor function for numerous signal transduction pathways, the phenotypical changes due to HS3ST2 reexpression were investigated in vitro using high and low invasive breast cancer cell lines. Compared to controls, highly invasive HS3ST2-expressing MDA-MB-231 cells showed enhanced Matrigel invasiveness, transendot ...[more]

PMID: 24752740

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Genes regulated by HS3ST2

Project description:We used Affymetrix HG U133 Plus 2.0 GeneChips to compare the transcriptome of HS3ST2-transfected and control vector-transfected MDA-MB-231 cells.

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