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Expression data from human Th1 and Th1Th17 cells

ABSTRACT: We previously demonstrated that Th1Th17 cells are highly permissive to HIV-1, whereas Th1 cells are relatively resistant. Here, we investigated molecular mechanisms underlying these differences. Superior HIV replication in Th1Th17 vs. Th1 cells was regulated by entry and post-entry mechanisms. We used microarrays to detail the gene expression signatures caracterizing Th1 cells from Th1Th17. Primary human Th1 (CXCR3+CCR6- phenotype) and Th1Th17 (CXCR3+CCR6- phenotype) CD4+ T-cells were isolated by flow cytometry from HIV-uninfected, healthy donors. Cells were stimulated via CD3/CD28 for 3 days. The RNA was extracted and hybridized on the GeneChipM-BM-. Human Genome U133 Plus 2.0 Array (Affymetrix).

ORGANISM(S): Homo sapiens

SUBMITTER: Jean-Philippe Goulet

PROVIDER: E-GEOD-50175 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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