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Genome-wide analysis of transcription factor PPARÎ³-responsive gene expression in the nodose ganglion (NG)

ABSTRACT: Analysis of gene expression regulated by PPARÎ³ in the nodose ganglion of Phox2b::Cre; PPARÎ³ fl/+ and Phox2b::Cre; PPARÎ³ fl/fl mice. Resutls demonstrate potential PPARÎ³ transcriptional targets in neurons RNA was purified from laser-captured nodose neurons. Three independent biological replicates were prepared by pooling RNA of nodose neurons from multiple animals of the same genotypes. Genomics and Microarray Core Facility at UT Southwestern ( http://microarray.swmed.edu/) checked RNA quality and performed the hybridization with a Mouse-6 V2 BeadChip (Illumina Inc.). We used Partek Genomics Suite 6.5 (Partek Inc.) and Ingenuity Pathway Analysis (Ingenuity Systems Inc.) for data and pathway analysis respectively.

ORGANISM(S): Mus musculus

SUBMITTER: Chen Liu

PROVIDER: E-GEOD-50404 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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