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Gene expression analysis of RB1 knockdown in bone in response to radiation

ABSTRACT: The role of RB1 in response to radiation was examined in human osteoblasts. We demonstrate that RB1 induced SASP genes, a response which was attenuated in RB1 knockdown osteoblasts. Subconfluent hOB shEV and shRB1 cells were irradiated at 4 Gy using a 137Cesium source at a dose rate of 1.7 min/Gy and cell pelllets collected 0, 2, 4, 8, 16 and 24 hrs after irradiation.

ORGANISM(S): Homo sapiens

SUBMITTER: Maya Kansara

PROVIDER: E-GEOD-50532 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Immune response to RB1-regulated senescence limits radiation-induced osteosarcoma formation.

Kansara Maya M Leong Huei San HS Lin Dan Mei DM Popkiss Sophie S Pang Puiyi P Garsed Dale W DW Walkley Carl R CR Cullinane Carleen C Ellul Jason J Haynes Nicole M NM Hicks Rod R Kuijjer Marieke L ML Cleton-Jansen Anne-Marie AM Hinds Philip W PW Smyth Mark J MJ Thomas David M DM

The Journal of clinical investigation 20131115 12

Ionizing radiation (IR) and germline mutations in the retinoblastoma tumor suppressor gene (RB1) are the strongest risk factors for developing osteosarcoma. Recapitulating the human predisposition, we found that Rb1+/- mice exhibited accelerated development of IR-induced osteosarcoma, with a latency of 39 weeks. Initial exposure of osteoblasts to carcinogenic doses of IR in vitro and in vivo induced RB1-dependent senescence and the expression of a panel of proteins known as senescence-associated ...[more]

PMID: 24231354

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