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Coordinated genomic control of ciliogenesis and cell movement by Rfx2

ABSTRACT: We have performed a systems-level analysis of the RFX/Daf-19 family transcription factor, Rfx2. Using a combination of high-throughput sequencing of Rfx2-regulated transcripts and chromosomal binding sites, we provide a comprehensive accounting of the target genes by which Rfx2 controls ciliogenesis and cilia beating in vertebrates. RNA-seq: two biological replicates for control and RFX2 knockdown by morpholino injection, ChIP-seq: RFX2-GFP pulldown with GFP antibody, GFP only expression used as control

ORGANISM(S): Xenopus laevis

SUBMITTER: Taejoon Kwon

PROVIDER: E-GEOD-50593 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Coordinated genomic control of ciliogenesis and cell movement by Rfx2

Project description:We have performed a systems-level analysis of the RFX/Daf-19 family transcription factor, Rfx2. Using a combination of high-throughput sequencing of Rfx2-regulated transcripts and chromosomal binding sites, we provide a comprehensive accounting of the target genes by which Rfx2 controls ciliogenesis and cilia beating in vertebrates.

2013-12-12 | GSE50593 | GEO

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Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes [RNA-Seq P21]

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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