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Gene expression from pericytes and endothelial cells

ABSTRACT: Aims: Vascular wall resident stem cells (SCs) hold great promise for cardiovascular regenerative therapy. This study evaluated the impact of oxidative stress on the viability and functionality of saphenous vein'derived pericytes (SVPs), SVPs'deriv ed cells and terminally differentiated vascular cells. We also investigated the molecular mechanisms underlying the SVP resistance to oxidative stress. Results: SVPs exhibit a significantly higher resistance to oxidative stress resulting in reduced apoptosis upon exposure to hydrogen peroxide in comparison to endothelial cells (ECs). This was attributed to upregulation of genes encoding for anti'oxidant enzymes, especially superoxide dismutases (SODs) and catalase. Pharmacological inhibition of SODs increased ROS levels in SVPs and impaired their survival. Furthermore, we confirm that, when exposed to differentiation stimuli, SVPs are able to generate mesodermic lineages. Interestingly, differentiation of SVPs resulted in SOD down'regulation and increased apoptosis upon exposure to hydrogen peroxide. Oxidative stress caused an incremental increase on SVPs migration, whilst being inhibitory for the ECs. Additionally, oxidative stress did not impair SVPs capacity to promote angiogenesis in vitro. Innovations: This study for the first time demonstrates that SCs resident in veins of cardiovascular patients are endowed with enhanced detoxifier and antioxidant system. Conclusions: SVPs expanded from vein remnants of coronary artery bypass graft surgery express antioxidant defense mechanisms which allow them resist to high levels of ROS. These properties highlight the potential of SVPs in cardiovascular regenerative medicine. 2 groups 3 replicates

ORGANISM(S): Homo sapiens

SUBMITTER: Klemens Vierlinger

PROVIDER: E-GEOD-50758 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression from pericytes and endothelial cells

Project description:Aims: Vascular wall resident stem cells (SCs) hold great promise for cardiovascular regenerative therapy. This study evaluated the impact of oxidative stress on the viability and functionality of saphenous vein'derived pericytes (SVPs), SVPs'deriv ed cells and terminally differentiated vascular cells. We also investigated the molecular mechanisms underlying the SVP resistance to oxidative stress. Results: SVPs exhibit a significantly higher resistance to oxidative stress resulting in reduced apoptosis upon exposure to hydrogen peroxide in comparison to endothelial cells (ECs). This was attributed to upregulation of genes encoding for anti'oxidant enzymes, especially superoxide dismutases (SODs) and catalase. Pharmacological inhibition of SODs increased ROS levels in SVPs and impaired their survival. Furthermore, we confirm that, when exposed to differentiation stimuli, SVPs are able to generate mesodermic lineages. Interestingly, differentiation of SVPs resulted in SOD down'regulation and increased apoptosis upon exposure to hydrogen peroxide. Oxidative stress caused an incremental increase on SVPs migration, whilst being inhibitory for the ECs. Additionally, oxidative stress did not impair SVPs capacity to promote angiogenesis in vitro. Innovations: This study for the first time demonstrates that SCs resident in veins of cardiovascular patients are endowed with enhanced detoxifier and antioxidant system. Conclusions: SVPs expanded from vein remnants of coronary artery bypass graft surgery express antioxidant defense mechanisms which allow them resist to high levels of ROS. These properties highlight the potential of SVPs in cardiovascular regenerative medicine.

2014-01-01 | GSE50758 | GEO

Transcription profiling of Saccharomyces cerevisiae reveals the Cbf1 basic helix-loop-helix transcription factor is required for the response to hydrogen peroxide stress

Project description:Cbf1 is a basic helix-loop-helix transcription factor which regulates the expression of many genes of the sulphur assimilation pathway in yeast. Gamma-glutamylcysteine synthetase (GSH1), encoding the rate-limiting enzyme in glutathione biosynthesis, is constitutively elevated in cbf1 mutants indicating that Cbf1 normally represses GSH1 expression. We used transcriptional profiling to show that a number of antioxidant and stress genes are similarly repressed by Cbf1, consistent with the high oxidant tolerance of cbf1 mutants. Our data indicate that Cbf1 plays a key role in the regulation of gene expression during oxidative stress conditions induced by exposure to hydrogen peroxide. The Yap1 transcription factor induces GSH1 expression in response to hydrogen peroxide in a mechanism that does not require the Met4 transcription factor to overcome the inhibitory action of Cbf1. We show that hydrogen peroxide does not affect Cbf1 occupancy on the GSH1 promoter, but results in specific modification of Cbf1 by phosphorylation. Our data indicate that casein kinase (CK2) can directly phosphorylate Cbf1 in vitro. Furthermore, CK2 activity is required to phosphorylate Cbf1 and induce GSH1 expression in yeast cells in response to hydrogen peroxide. CK2 mutants are sensitive to hydrogen peroxide consistent with a role for CK2 in regulating the cellular response to oxidative stress. CK2 is a ubiquitous serine/threonine protein kinase and the finding that it is regulated by oxidative stress is particularly interesting, since there is increasing evidence that it is involved in a number of pathological conditions including cancer, neurodegenerative and cardiovascular diseases.

2009-10-01 | E-MEXP-2159 | biostudies-arrayexpress

Response of Bacillus pumilus to hydrogen peroxide

Project description:Reactive oxygen species such as hydrogen peroxide occur in all aerobically living organisms. Oxidative stress during fermentation can impair the fitness of the production host and the quality of the product. B. pumilus has been described as highly resistant to hydrogen peroxide. The response of exponentially growing B. pumilus cells to hydrogen peroxide was studied. Two-condition experiment, unstressed versus hydrogen peroxide stressed cells, 3 biological replicates

2014-05-17 | E-GEOD-57735 | biostudies-arrayexpress

Transcription profiling of Pseudomonas aeruginosa treated with hydrogen peroxide

Project description:Background: Pseudomonas aeruginosa, a pathogen infecting those with cystic fibrosis, encounters toxicity from phagocyte-derived reactive oxidants including hydrogen peroxide during active infection. P. aeruginosa responds with adaptive and protective strategies against these toxic species to effectively infect humans. Despite advances in our understanding of the responses to oxidative stress in many specific cases, the connectivity between targeted protective genes and the rest of cell metabolism remains obscure. Results: Herein, we performed a genome-wide transcriptome analysis of the cellular responses to hydrogen peroxide in order to determine a more complete picture of how oxidative stress-induced genes are related and regulated. Our data reinforce the previous conclusion that DNA repair proteins and catalases may be among the most vital antioxidant defense systems of P. aeruginosa. Our results also suggest that sublethal oxidative damage reduces active and/or facilitated transport and that intracellular iron might be a key factor for a relationship between oxidative stress and iron regulation. Perhaps most intriguingly, we revealed that the transcription of all F-, R-, and S-type pyocins was upregulated by oxidative stress and at the same time, a cell immunity protein (pyocin S2 immunity protein) was downregulated, possibly leading to self-killing activity. Conclusions: This finding proposes that pyocin production might be another novel defensive scheme against oxidative attack by host cells. Experiment Overall Design: We conducted four and five independent microarray experiments with biological replicates in the absence (control) and the presence (experimental) of hydrogen peroxide, respectively.

2007-07-19 | E-GEOD-3090 | biostudies-arrayexpress

Metal resistant bacterium Cupriavidus metallidurans CH34

2014-09-13 | GSE24094 | GEO

Response of Bacillus pumilus to hydrogen peroxide

2014-05-17 | GSE57735 | GEO

Metal resistant bacterium Cupriavidus metallidurans CH34

Project description:Metal resistant bacterium Cupriavidus metallidurans CH34 uses non-conventional proteins to combat oxidative stress Keywords: Cupriavidus metallidurans; hydrogen peroxide; oxidative stress; Two-condition experiments. Comparing samples after challenging with oxidative stress versus non-induced samples. Biological triplicate. Each array contains 3 technical replicates.

2014-09-13 | E-GEOD-24094 | biostudies-arrayexpress

Gene expression changes in response to aging compared to heat stress, oxidative stress and ionizing radiation in Drosophila melanogaster

Project description:Gene expression changes in response to aging, hyperoxia, hydrogen peroxide, ionizing radiation, and heat stress were compared using microarrays. While aging shared features with each stress, aging was more similar to the stresses most associated with oxidative stress (hydrogen peroxide, hyperoxia, ionizing radiation) than to heat stress. Aging is associated with down-regulation of numerous mitochondrial genes, including electron-transport-chain (ETC) genes and mitochondrial metabolism genes, and a sub-set of these changes was also observed upon hydrogen peroxide stress and ionizing radiation stress. Aging shared the largest number of gene expression changes with hyperoxia. The extensive down-regulation of mitochondrial and ETC genes during aging is consistent with an aging-associated failure in mitochondrial maintenance, which may underlie the oxidative stress-like and proteotoxic stress-like responses observed during aging.

2012-11-19 | GSE42255 | GEO

Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942

Project description:An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the ascorbate-glutathione cycle, regulate redox status in cells to guard against such damage. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzes the glutathione-dependent reduction of oxidized ascorbate (dehydroascorbate) and contains a redox active site and glutathione binding-site. The DHAR gene is important in biological and abiotic stress responses involving reduction of the oxidative damage caused by ROS. In this study, a transgenic microalgae (TA) strain was constructed by cloning the Oryza sativa L. japonica DHAR (OsDHAR) gene controlled by an isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter (Ptrc) into the Synechococcus elongatus PCC 7942 strain of cyanobacteria to study the functional activities of OsDHAR under oxidative stress caused by hydrogen peroxide exposure. OsDHAR expression increased the growth of S. elongatus PCC 7942 under oxidative stress by reducing the levels of hydroperoxides and malondialdehyde (MDA) and mitigating the loss of chlorophyll. DHAR and glutathione S-transferase activity were higher than in the wild-type (WT) strain. Additionally, overexpression of OsDHAR in S. elongatus PCC 7942 greatly increased the glutathione (GSH)/glutathione disulfide (GSSG) ratio compared to ascorbic acid (AsA)/dehydroascorbate (DHA) ratio in the presence or absence of hydrogen peroxide. These results strongly suggest that DHAR attenuates deleterious oxidative effects via the glutathione (GSH)-dependent antioxidant system in cyanobacterial cells. The expression of heterologous OsDHAR in S. elongatus PCC 7942 protected cells from oxidative damage through a GSH-dependent antioxidant system via GSH-dependent reactions at the redox active site and GSH binding site residues during oxidative stress.

2019-11-25 | GSE140121 | GEO

Time course of oxidative stress responses in yeast, caused by Menadione or hydrogen peroxide

Project description:Oxidative stress caused by Menadione or Hydrogen peroxide in synchronized Saccharomyces cerevisiae cultures. Alpha factor synchronized cultures (0.2-0.4 OD), treated at the beginning of S phase (25 min after release from G1 arrest) with either 2 mM Menadione (MD) or 0.24 mM Hydrogen peroxide (HP), show cell cycle effects. Cells treated with MD arrested at G1. Cells treated with HP delayed at S and then, after removal of HP at 135 minutes , continued the cell cycle, only to arrest at G2/M. Growth was carried out in 30C with shaking (295 rpm). Two time course experiments were performed with each oxidative stress agent, designated as H2O2 and H2O2_II, MD and MD_II. Keywords = oxidative stress Keywords = menadione Keywords = hydrogen peroxide Keywords = time course Keywords = cell cycle Keywords = yeast

2005-02-04 | E-GEOD-2239 | biostudies-arrayexpress

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