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Gene expression analysis of PBMC from HIV and HIV/TB co-infected patients

ABSTRACT: Diagnosis of TB, especially in the presence of an HIV co-infection, can be challenging when using conventional diagnostic methods. In this study, we analyzed global gene expression data from PBMC of patients that were either mono-infected with HIV or co-infected with HIV and TB in order to identify a TB-specific gene signature. Total RNA obtained from PBMC from a South African cohort. Microarry analysis was performed to compare gene expression in patients either infected with HIV or co-infected with HIV/TB.

ORGANISM(S): Homo sapiens

SUBMITTER: Louise Showe

PROVIDER: E-GEOD-50834 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of a 251 gene expression signature that can accurately detect M. tuberculosis in patients with and without HIV co-infection.

Dawany Noor N Showe Louise C LC Kossenkov Andrew V AV Chang Celia C Ive Prudence P Conradie Francesca F Stevens Wendy W Sanne Ian I Azzoni Livio L Montaner Luis J LJ

PloS one 20140225 2

<h4>Background</h4>Co-infection with tuberculosis (TB) is the leading cause of death in HIV-infected individuals. However, diagnosis of TB, especially in the presence of an HIV co-infection, can be limiting due to the high inaccuracy associated with the use of conventional diagnostic methods. Here we report a gene signature that can identify a tuberculosis infection in patients co-infected with HIV as well as in the absence of HIV.<h4>Methods</h4>We analyzed global gene expression data from peri ...[more]

PMID: 24587128

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