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DNA methylation analysis of PBMC from HIV and HIV/TB co-infected patients

ABSTRACT: Genome wide DNA methylation profiling of PBMC from South African patients either infected with HIV only or coinfected with HIV and tuberculosis (TB). The Illumina Infinium 27k Human DNA methylation Beadchip was used to obtain DNA methylation profiles from PBMC samples. Samples included 19 HIV patients and 20 HIV/TB co-infected patients. Bisulphite converted DNA were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2

ORGANISM(S): Homo sapiens

SUBMITTER: Louise Showe

PROVIDER: E-GEOD-50835 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of a 251 gene expression signature that can accurately detect M. tuberculosis in patients with and without HIV co-infection.

Dawany Noor N Showe Louise C LC Kossenkov Andrew V AV Chang Celia C Ive Prudence P Conradie Francesca F Stevens Wendy W Sanne Ian I Azzoni Livio L Montaner Luis J LJ

PloS one 20140225 2

<h4>Background</h4>Co-infection with tuberculosis (TB) is the leading cause of death in HIV-infected individuals. However, diagnosis of TB, especially in the presence of an HIV co-infection, can be limiting due to the high inaccuracy associated with the use of conventional diagnostic methods. Here we report a gene signature that can identify a tuberculosis infection in patients co-infected with HIV as well as in the absence of HIV.<h4>Methods</h4>We analyzed global gene expression data from peri ...[more]

PMID: 24587128

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DNA methylation analysis of PBMC from HIV and HIV/TB co-infected patients

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