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MRNA profiles of activated and resting T cells and their exosomes

ABSTRACT: Microarray analysis of exosomal and cellular mRNAs in activated and resting primary human T lymphoblasts Primary human T lymphoblasts were obtained from eight donors and total RNA was isolated from cells and derived exosomes before and after activation with PMA plus ionomycin.

ORGANISM(S): Homo sapiens

SUBMITTER: VIllaroya Carolina

PROVIDER: E-GEOD-50971 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs.

Villarroya-Beltri Carolina C Gutiérrez-Vázquez Cristina C Sánchez-Cabo Fátima F Pérez-Hernández Daniel D Pérez-Hernández Daniel D Vázquez Jesús J Martin-Cofreces Noa N Martinez-Herrera Dannys Jorge DJ Pascual-Montano Alberto A Mittelbrunn María M Sánchez-Madrid Francisco F

Nature communications 20130101

Exosomes are released by most cells to the extracellular environment and are involved in cell-to-cell communication. Exosomes contain specific repertoires of mRNAs, microRNAs (miRNAs) and other non-coding RNAs that can be functionally transferred to recipient cells. However, the mechanisms that control the specific loading of RNA species into exosomes remain unknown. Here we describe sequence motifs present in miRNAs that control their localization into exosomes. The protein heterogeneous nuclea ...[more]

PMID: 24356509

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microRNA profiles of activated and resting T cells and their exosomes

Project description:Microarray analysis of exosomal and cellular miRNAs in activated and resting primary human T lymphoblasts Primary human T lymphoblasts were obtained from eight donors and total RNA was isolated from cells and derived exosomes before and after activation with PMA plus ionomycin.

2014-02-27 | E-GEOD-50953 | biostudies-arrayexpress

mRNA and miRNA profiles of activated and resting T cells and their exosomes

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-02-27 | E-GEOD-50972 | biostudies-arrayexpress

microRNA profiles of immune cells and their exosomes

Project description:Microarray analysis of exosomal miRNAs vs the miRNAs of their respective donor cells. To determine the miRNA repertoires of exosomes secreted by immune cells, we isolated exosomes from cell supernatants of the Raji B cell line, the Jurkat-derived J77 T cell line, and primary dendritic cells (DCs) derived from human monocytes. Exosomes were isolated by a series of microfiltration and ultracentrifugation steps

2011-03-17 | E-GEOD-27997 | biostudies-arrayexpress

Exosome from mouse T lymphocyte cells

Project description:Exosomes transferred from the T cell to the APC contain many different types of biologically active molecules, including proteins and genetic material. To investigate the possible specific function of the different components of the biologic material transferred from the T cell to the DC, we have characterized the protein and genetic content of EVs isolated from the culture supernatant of primary T lymphoblasts by differential ultracentrifugation

2018-05-30 | PXD008843 | Pride

X chromosome cDNA microarray screen of lymphoblasts from males with XLMR

Project description:The goal of this study is to identify genes that show altered transcript levels in lymphoblasts from male patients with XLMR as candidate genes that are responsible for XLMR. Keywords: disease state Using a custom X chromosome cDNA microarray, we studied X chromosome gene expression in lymphoblasts from 43 patients with XLMR and identified those genes with altered transcript levels as compared to that in 6 normal controls.

2010-06-11 | E-GEOD-7470 | biostudies-arrayexpress

Analyses of long-range interactions of the NR0B1 (DAX1) promoter using chromosome conformation capture-on-chip

Project description:Heterozygous point mutations or genomic deletions of NR0B1 in Xp21.2 result in congenital adrenal hypoplasia and hypogonadotropic hypogonadism, whereas the NR0B1 locus duplications in XY individuals lead to gonadal dysgenesis and a male-to-female dosage-sensitive sex reversal. We previously reported an ~ 257 kb deletion mapping 11 kb upstream to NR0B1 in a XY female with primary amenorrhea, small immature uterus, and gonadal dysgenesis pointing to an alteration of its regulatory region. To identify the potential regulatory elements of NR0B1, we have analyzed its 2 Mb flanking regions using chromosome conformation capture-on-chip (4C) in Sertoli cells and lymphoblasts. We confirm the involvement of the previously proposed regulatory region in the control of NR0B1 expression and describe several novel potential chromatin interactions within the NR0B1 locus that may be involved in sex differentiation. Custom designed 3x720K tiling microarrays covering 4 Mb region (chrX:28,082,100-32,087,100) flanking NR0B1 gene of interest

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Chromosome conformation capture-on-chip analysis of long-range cis interactions of the SOX9 promoter

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Exosomes miRNA array submission

Project description:MicroRNA microarray profilling analysis was performed on exosomes derived from 4T1 mammary epithelial cancer cells in two culture conditions, hypoxia and normoxia.

2014-06-01 | E-MTAB-2361 | biostudies-arrayexpress

Profiling of endogenous and exosomal microRNAs in colon cancer

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-04-01 | E-GEOD-40247 | biostudies-arrayexpress

FOXF1 promoter-putative enhancer chromatin looping

Project description:To determine if there is a physical interaction between the FOXF1 promoter and putative enhancer sequences ~250kb upstream of the promoter chromosome conformation capture-on-chip (4C) analysis was performed. An unanticipated and tremendous amount of the non-coding sequences of the human genome are transcribed. Long non-coding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides and their functions remain enigmatic. We demonstrate that deletions of lncRNA genes cause a lethal lung developmental disorder, Alveolar Capillary Dysplasia with Misalignment of Pulmonary Veins (ACD/MPV), with parent of origin effects. We identify non-coding overlapping deletions 250 kb upstream to FOXF1 in nine patients with ACD/MPV that arose de novo specifically on the maternally inherited chromosome and delete a fetal lung-specific EST, part of an lncRNA. These deletions define distant cis-regulatory region that harbors a differentially methylated CpG island, binds GLI2 depending on the methylation status of this CpG island, and physically interacts with and up-regulates the FOXF1 promoter, consistent with the absence of the fetal lung-transcribed lncRNA perturbing FOXF1 regulation. LncRNA-mediated chromatin interactions may be responsible for position effect phenomenon and potentially cause many disorders of human development. 4C analysis using 16q24.1 specific 3x720K arrays demonstrated physical interaction between the FOXF1 promoter and distant putative regulatory sequences, about 250 kb upstream in human pulomonary microvascular endothelial cells; 2 biological replicates performed; this chromatin looping was not detected in lymphoblasts that do not express FOXF1 and hence serve as a negative control.

2012-07-10 | E-GEOD-39256 | biostudies-arrayexpress

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