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Gamma-secretase inhibitor plus fludarabine in CLL

ABSTRACT: Combination of GSI with fludarabine has a synergistic antileukemic effect in primary NOTCH1-mutated CLL cells We used microarrays to detail the mechanism of synergy of GSI and fludarabine combination in NOTCH1-mutated CLL cells Global RNA expression in CLL primary cells treated with GSI, fludarabine and the combination at 48 hours of treatment

ORGANISM(S): Homo sapiens

SUBMITTER: DOLORS COLOMER

PROVIDER: E-GEOD-51044 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gamma-secretase inhibitor plus fludarabine in CLL

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Project description:Our data demonstrate binding of NOTCH1 on the promoters of HSF1 and HSF1 targets. We performed RNA-seq upon control conditions, inhibition of the NOTCH1 pathway by gSI, HSF1 knockdown and combination of HSF1 knockdown and gSI treatment. Our results show that NOTCH1 regulates the expression of heat shock response genes. Moreover, combination of HSF1 downregulation and gSI treatment further decreased the expression of heat shock response genes compared to single treatments.

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