Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional responses of murine macrophages to the adenylate cyclase toxin of Bordetella pertussis

ABSTRACT: Three different recombinant forms of CyaA were used to investigate transcriptional responses of murine bone marrow-derived macrophages (BMDMs) using Affymetrix Mouse Genome Genechips. These forms were enzymically active, invasive CyaA, nonenzymically active, invasive CyaA (CyaA*) and non-enzymically active, non-invasive CyaA (proCyaA*). BMMs, treated with 20 ng/ml of CyaA for 24 h, showed over 1000 significant changes in gene transcription compared with control cells. CyaA caused an increase in transcription of many inflammatory genes and genes associated with various signalling cascades such as those involved in cyclic AMP-dependent protein kinase A signalling. Most strikingly, CyaA caused down-regulation of numerous genes involved in cell proliferation. CyaA* at 20 ng/ml significantly up-regulated the transcription of only twelve genes after 24 h whereas proCyaA* at this concentration significantly increased the transcription of only two genes. The effect of administration of three different recombinant forms of pertussis toxin to mouse bone marrow derived macrophages are compared with a vehicle only contol 24 hours after treatment in technical triplicate measurements

ORGANISM(S): Mus musculus

SUBMITTER: Nigel Binns

PROVIDER: E-GEOD-51075 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional responses of murine macrophages to the adenylate cyclase toxin of Bordetella pertussis.

Cheung Gordon Y C GY Dickinson Paul P Sing Garwin G Craigon Marie M Ghazal Peter P Parton Roger R Coote John G JG

Microbial pathogenesis 20070814 1

Three different recombinant forms of CyaA were used to investigate transcriptional responses of murine bone marrow-derived macrophages (BMMs) using Affymetrix Mouse Genome GeneChips. These forms were enzymically active, invasive CyaA, non-enzymically active, invasive CyaA (CyaA*) and non-enzymically active, non-invasive CyaA (proCyaA*). BMMs, treated with 20 ng/ml of CyaA for 24h, showed over 1000 significant changes in gene transcription compared with control cells. CyaA caused an increase in t ...[more]

PMID: 17890046

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Dataset Information

Transcriptional responses of murine macrophages to the adenylate cyclase toxin of Bordetella pertussis

Publications

Transcriptional responses of murine macrophages to the adenylate cyclase toxin of Bordetella pertussis.

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