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Analyses of the chromatin and transcriptional basis for lateral inhibition in isolated intestinal epithelial cells.

ABSTRACT: We analyzed chromatin modifications, DNaseI-hypersensitive sites, and occupancy of a key secretory-lineage transcription factor, ATOH1. We found that lateral inhibition in the intestine occurs through ATOH1 exerting direct control within a broadly permissive chromatin state that is established in stem cells and is highly similar in specified progenitors of divergent potential. Mapping chromatin modifications (H3K4me2 and H3K27ac), DNaseI hypersensitivity (DHS), and ATOH1 binding sites in isolated intestinal crypt progenitors and mature intestinal villus cells.

ORGANISM(S): Mus musculus

SUBMITTER: Fugen Li

PROVIDER: E-GEOD-51458 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Broadly permissive intestinal chromatin underlies lateral inhibition and cell plasticity.

Kim Tae-Hee TH Li Fugen F Ferreiro-Neira Isabel I Ho Li-Lun LL Luyten Annouck A Nalapareddy Kodandaramireddy K Long Henry H Verzi Michael M Shivdasani Ramesh A RA

Nature 20140112 7489

Cells differentiate when transcription factors bind accessible cis-regulatory elements to establish specific gene expression programs. In differentiating embryonic stem cells, chromatin at lineage-restricted genes becomes sequentially accessible, probably by means of 'pioneer' transcription factor activity, but tissues may use other strategies in vivo. Lateral inhibition is a pervasive process in which one cell forces a different identity on its neighbours, and it is unclear how chromatin in equ ...[more]

PMID: 24413398

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