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Genome-wide incorporation dynamics reveal distinct categories of turnover for the histone variant H3.3


ABSTRACT: We developed a system to study the DNA replication-independent turnover nucleosomes containing the histone variant H3.3 in mammalian cells. By measuring the genome-wide incorporation of H3.3 at different time points following epitope-tagged H3.3 expression, we find three categories of H3.3-nucleosome turnover in vivo: rapid turnover, intermediate turnover and, specifically at telomeres, slow turnover. Our data indicate that H3.3-containing nucleosomes at enhancers and promoters undergo a rapid turnover that is associated with active histone modification marks including H3K4me1, H3K4me3, H3K9ac, H3K27ac and the histone variant H2A.Z. The rate of turnover is negatively correlated with H3K27me3 at regulatory regions and with H3K36me3 at gene bodies. Examination of incorporation dynamics of histone variant H3.3

ORGANISM(S): Mus musculus

SUBMITTER: Wenfei Jin 

PROVIDER: E-GEOD-51505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide incorporation dynamics reveal distinct categories of turnover for the histone variant H3.3.

Kraushaar Daniel C DC   Jin Wenfei W   Maunakea Alika A   Abraham Brian B   Ha Misook M   Zhao Keji K  

Genome biology 20130101 10


<h4>Background</h4>Nucleosomes are present throughout the genome and must be dynamically regulated to accommodate binding of transcription factors and RNA polymerase machineries by various mechanisms. Despite the development of protocols and techniques that have enabled us to map nucleosome occupancy genome-wide, the dynamic properties of nucleosomes remain poorly understood, particularly in mammalian cells. The histone variant H3.3 is incorporated into chromatin independently of DNA replication  ...[more]

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