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Identification of direct targets of Arabidopsis JAGGED in floral buds

ABSTRACT: JAGGED (JAG) is a regulatory gene that controls shoot organ growth in Arabidopsis. To reveal the molecular links between JAG and the cellular activities required for tissue growth, we used chromatin immunoprecipitation-high-throughput sequencing (ChIP-Seq). To reveal the genome-wide JAG binding sites, we used anti-GFP antibodies to pull down JAG-bound DNA from jag-2 inflorescences complemented with a genomic JAG-GFP fusion (JAG:JAG-GFP) [14]. ChIP-Seq was performed and analyzed in triplicate, with wild-type inflorescences used as epitope-negative controls.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Robert Sablowski

PROVIDER: E-GEOD-51537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Arabidopsis JAGGED links floral organ patterning to tissue growth by repressing Kip-related cell cycle inhibitors.

Schiessl Katharina K Muiño Jose M JM Sablowski Robert R

Proceedings of the National Academy of Sciences of the United States of America 20140204 7

Plant morphogenesis requires coordinated cytoplasmic growth, oriented cell wall extension, and cell cycle progression, but it is debated which of these processes are primary drivers for tissue growth and directly targeted by developmental genes. Here, we used ChIP high-throughput sequencing combined with transcriptome analysis to identify global target genes of the Arabidopsis transcription factor JAGGED (JAG), which promotes growth of the distal region of floral organs. Consistent with the role ...[more]

PMID: 24497510

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